Optimization of multiplexed CRISPR/Cas9 system for highly efficient genome editing inSetaria viridis

Weiss, Trevor; Wang, Chunfang; Kang, Xiaojun; Crisp, Peter A.; Zhou, Peng; Springer, Nathan M.; Zhang, Feng; Weiss, Trevor; Wang, Chunfang; Kang, Xiaojun; Elena Gamo, Maria; Starker, Colby G.; Crisp, Peter A.; Zhou, Peng; Springer, Nathan M.; Voytas, Daniel F.; Zhang, Feng; Weiss, Trevor; Wang, Chunfang; Kang, Xiaojun; Elena Gamo, Maria; Starker, Colby G.; Crisp, Peter A.; Zhou, Peng; Springer, Nathan M.; Voytas, Daniel F.; Zhang, Feng; Weiss, Trevor; Wang, Chunfang; Kang, Xiaojun; Elena Gamo, Maria; Starker, Colby G.; Voytas, Daniel F.; Zhang, Feng; Zhao, Hui; Zhao, Hui; Elena Gamo, Maria; Starker, Colby G.; Voytas, Daniel F.; Crisp, Peter A.; SCI

机构： Univ Minnesota, Dept Plant & Microbial Biol, Coll Biol Sci, Minneapolis, MN 55108 USA; Univ Minnesota, Ctr Plant Precis Genom, Minneapolis, MN 55108 USA; Univ Minnesota, Microbial & Plant Genom Inst, Minneapolis, MN 55108 USA; Univ Minnesota, Ctr Genome Engn, Minneapolis, MN 55108 USA; Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou 571101, Hainan, Peoples R China; Chinese Acad Trop Agr Sci, Hainan Key Lab Biosafety Monitoring & Mol Breedin, Haikou 571101, Hainan, Peoples R China; Univ Minnesota, Dept Genet Cell Biol & Dev, Minneapolis, MN 55108 USA; Univ Queensland, Sch Agr & Food Sci, Brisbane, Qld 4072, Australia;

关键词： Cas9; CRISPR; multiplexed genome editing; NHEJ and MMEJ DNA repair; Setaria viridis; Trex2exonuclease; technical advance

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Transcriptional mechanism of differential sugar accumulation in pulp of two contrasting mango (Mangifera indica L.) cultivars

Li, Li; Wu, Hong-Xia; Ma, Xiao-Wei; Xu, Wen-Tian; Liang, Qing-Zhi; Wang, Song-Biao; Zhan, Ru-Lin; SCI

机构： Chinese Acad Trop Agr Sci, South Subtrop Crops Res Inst, Minist Agr, Key Lab Trop Fruit Biol, Zhanjiang 524091, Peoples R China; Chinese Acad Trop Agr Sci, Haikou Expt Stn, Inst Trop Fruit Tree, Haikou 571101, Hainan, Peoples R China;

关键词： RNA-seq; Sugar metabolism; Mango; D-glucose; Alpha-glucosidase; Invertase; Alpha-amylase

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The Nutrient Status of Plant Roots Reveals Competition Intensities in Rubber Agroforestry Systems

Wu, Junen; Zeng, Huanhuan; Zhao, Fan; Chen, Chunfeng; Jiang, Xiaojin; Zhu, Xiai; Wang, Pingyuan; Liu, Wenjie; Zeng, Huanhuan; Zhao, Fan; Chen, Chunfeng; Jiang, Xiaojin; Zhu, Xiai; Wang, Pingyuan; Liu, Wenjie; Zeng, Huanhuan; Wu, Zhixiang; SCI

机构： Yunnan Normal Univ, Fac Geog, Kunming 650050, Yunnan, Peoples R China; Chinese Acad Sci, Xishuangbanna Trop Bot Garden, CAS Key Lab Trop Forest Ecol, Menglun 666303, Peoples R China; Ctr Plant Ecol, Core Bot Gardens, Menglun 666303, Peoples R China; Univ Chinese Acad Sci, Beijing 100049, Peoples R China; Chinese Acad Trop Agr Sci, Rubber Res Inst, Danzhou 571737, Peoples R China;

关键词： agroforestry systems; Bayesian network; belowground competition; nutrient cycling; plant nutrient allocation; plant– soil interactions

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BcpLH organizes a specific subset of microRNAs to form a leafy head in Chinese cabbage (Brassica rapa ssp. pekinensis)

Ren, Wenqing; Wu, Feijie; Bai, Jinjuan; Li, Xiaorong; Yang, Xi; Xue, Wanxin; Liu, Heng; He, Yuke; Ren, Wenqing; Liu, Heng; SCI

机构： Chinese Acad Sci, Shanghai Inst Plant Physiol & Ecol, Natl Lab Plant Mol Genet, Fenglin Rd 300, Shanghai 200032, Peoples R China; Chinese Acad Sci, Grad Sch, Shanghai 200032, Peoples R China; Chinese Acad Trop Agr Sci, South Subtrop Crop Res Inst, Minist Agr, Zhanjiang, Guangdong, Peoples R China;

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Genome-Wide Analysis of the PIN Auxin Efflux Carrier Gene Family in Coffee

Huang, Xing; He, Chunping; Lu, Ying; Wu, Weihuai; Yi, Kexian; Huang, Xing; He, Chunping; Lu, Ying; Wu, Weihuai; Yi, Kexian; Huang, Xing; He, Chunping; Lu, Ying; Wu, Weihuai; Yi, Kexian; Bai, Xuehui; Guo, Tieying; Xie, Zhouli; Laimer, Margit; Du, Dengxiang; Gbokie, Thomas; Zhang, Zhirun; SCI

机构： Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Haikou 571101, Hainan, Peoples R China; Minist Agr & Rural Affairs, Key Lab Integrated Pest Management Trop Crops, Haikou 571101, Hainan, Peoples R China; Hainan Key Lab Monitoring & Control Trop Agr Pest, Haikou 571101, Hainan, Peoples R China; Dehong Trop Agr Res Inst Yunnan, Ruili 678600, Peoples R China; Peking Univ, Sch Life Sci, Beijing 100871, Peoples R China; Univ Nat Resources & Life Sci, Dept Biotechnol, Plant Biotechnol Unit, BOKU VIBT, A-1190 Vienna, Austria; Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China; Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Peoples R China; Coffee Engn Res Ctr China, Mangshi 678400, Peoples R China;

关键词： PIN; auxin efflux carrier; phylogeny; expression; Coffea arabica; Coffea canephora; Coffea eugenioides

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Lipopeptide Epimers and a Phthalide Glycerol Ether with AChE Inhibitory Activities from the Marine-Derived Fungus Cochliobolus Lunatus SCSIO41401

Dai, Yu; Li, Kunlong; She, Jianglian; Liao, Shengrong; Lin, Xiuping; Yang, Bin; Wang, Junfeng; Zhou, Xuefeng; Liu, Yonghong; Li, Kunlong; Liao, Shengrong; Lin, Xiuping; Yang, Bin; Wang, Junfeng; Zhou, Xuefeng; Liu, Yonghong; Zeng, Yanbo; Wang, Hao; Dai, Haofu; Tao, Huaming; Liu, Yonghong; SCI

机构： Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Peoples R China; Southern Marine Sci & Engn Guangdong Lab Guangzho, Guangzhou 511458, Peoples R China; Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Res & Dev Nat Prod Li Folk Med, Haikou 571101, Hainan, Peoples R China; Southern Med Univ, Sch Tradit Chinese Med, Guangzhou 510515, Peoples R China; Shenyang Pharmaceut Univ, Wuya Coll Innovat, Shenyang 110016, Peoples R China;

关键词： lipopeptides; phthalide; Cochliobolus lunatus; absolute configurations; AChE inhibitory

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Cassava breeding and agronomy in Asia: 50 years of history and future directions

Malik, Al Imran; Youabee, Laothao; Kongsil, Pasajee; Kittipadakul, Piya; Aiemnaka, Pornsak; Vu Anh Nguyen; Utsumi, Yoshinori; Seki, Motoaki; Tokunaga, Hiroki; Nguyen, Hai Anh; Van Dong Nguyen; Le Huy Ham; Ishitani, Manabu; Ou, Wenjun; Lu, Cheng; Chen, Songbi; Wang, Wenquan; Li, Kaimian; Sholihin; Srean, Pao; Sheela, M. N.; Lopez-Lavalle, Luis Augusto Becerra; Ceballos, Hernan; Gomez, Michael Selvaraj; Labarta, Ricardo; Ishitani, Manabu; Utsumi, Yoshinori; Seki, Motoaki; Tokunaga, Hiroki; Hu'u Hy Nguyen; Trong Hien Nguyen; Amawan, Suwaluk; Sok, Sophearith; SCI

机构： Int Ctr Trop Agr CIAT Laos, Lao PDR Off, Ban Nongviengkham, Vientiane, Laos; Kasetsart Univ, Fac Agr, Dept Agron, 50 Ngam Wong Wan Rd, Chatuchak Bangkok 10900, Thailand; Agr Genet Inst, Int Lab Cassava Mol Breeding, Natl Key Lab Plant Cell Biotechnol, Pham Van Dong Rd, Hanoi, Vietnam; Chinese Acad Trop Agr Sci CATAS, Haikou 571737, Hainan, Peoples R China; Indonesian Legume & Tuber Crops Res Inst, Kendalpayak Km 8,POB 66, Malang 65101, Indonesia; Univ Battambang, Fac Agr & Food Proc, Battambang, Cambodia; Cent Tuber Crops Res Inst Sreekariyam, Thiruvananthapuram 605017, Kerala, India; Int Ctr Trop Agr CIAT, Km 17,Recta Cali Palmira Apartado Aereo 6713, Cali, Colombia; RIKEN Ctr Sustainable Resource Sci, 1-7-22 Suehiro Cho, Yokohama, Kanagawa 2300045, Japan; Hung Loc Agr Res Ctr, Inst Agr Southern Vietnam, 121 Nguyen Binh Khiem,Dist 1, Hcm City, Vietnam; Root & Tuber Crop Res & Dev Ctr, Food & Field Crop Res Inst, Hanoi, Vietnam; Rayong Field Crops Res Ctr, Sukumvit Rd, Meang 21150, Rayong, Thailand; Int Ctr Trop Agr CIAT Asia, Phnom Penh, Cambodia;

关键词： Asia; cassava; conventional breeding; agronomy; new breeding techniques; data-driven agriculture; CMD

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Glutaredoxins Play an Important Role in the Redox Homeostasis and Symbiotic Capacity of Azorhizobium caulinodans ORS571

Cao, Yajun; Li, Mingxu; Fang, Xingxing; Zhu, Dan; Qiu, Wei; Zhu, Juanjuan; Zhong, Zengtao; Zhu, Jun; Jiang, Gaofei; Xu, Yangchun; Yu, Daogeng; SCI

机构： Nanjing Agr Univ, Coll Life Sci, Dept Microbiol, Nanjing 210095, Peoples R China; Nanjing Agr Univ, Natl Engn Res Ctr Organ Based Fertilizers, Jiangsu Collaborat Innovat Ctr Solid Organ Waste, Jiangsu Prov Key Lab Organ Solid Waste Utilizat,P, Nanjing 210095, Peoples R China; Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Danzhou 571737, Hainan, Peoples R China;

关键词： Azorhizobium caulinodans; glutaredoxin; ROS; symbiosis

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Multigene engineering of medium-chain fatty acid biosynthesis in transgenicArabidopsis thalianaby aCre/LoxPmultigene expression system

Zheng, Yusheng; Chen, Lizhi; Zhu, Zhiyong; Li, Dongdong; Zhou, Peng; SCI

机构： Hainan Univ, Coll Trop Crops, Haikou 570228, Hainan, Peoples R China; Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou 571101, Hainan, Peoples R China;

关键词： Medium-chain fatty acids (MCFAs); CnKASI; CnFatB3; CnLPAAT-B; Combinatorial expression effect; Cre; loxcombination system

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Identification and Characterization of Glycoproteins and Their Responsive Patterns upon Ethylene Stimulation in the Rubber Latex

Yu, Li; Yuan, Boxuan; Xie, Quanliang; Wang, Xuchu; Yu, Li; Yuan, Boxuan; Wang, Lingling; Ding, Guohua; Souleymane, Ousmane Ahmat; Zhang, Xueyan; Wang, Xuchu; Sun, Yong; SCI

机构： Shihezi Univ, Coll Life Sci, Shihezi 832003, Peoples R China; Hainan Normal Univ, Coll Life Sci, Key Lab Ecol Trop Isl, Minist Educ, Haikou 571158, Hainan, Peoples R China; Chinese Acad Trop Agr Sci, Rubber Res Inst, Danzhou 571737, Peoples R China;

关键词： comparative proteomics; Hevea brasiliensis; Hydroxymethylglutaryl-CoA synthase; glycosylated proteins; natural rubber biosynthesis; rubber latex

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