表达小反刍兽疫病毒H基因的山羊痘病毒通用转移载体的构建及鉴定(英文)

文献类型： 中文期刊

第一作者： 邵长春

作者： 邵长春;张强;吴国华;颜新敏;李健;王建科;卢晓丽;赵志荀;崔丽凡;高世功

作者机构：

期刊名称： Agricultural Science & Technology

ISSN： 1009-4229

年卷期： 2009 年 03 期

页码： 21-24+41

摘要： [Objective] This study was to develop a live vector vaccine of goat pox virus of Peste des petits ruminants(PPR). [Method] Using PCR amplification technique, PPR H gene was obtained, then ligated into pGEM-T easy vector; the recombinants were digested by Nhe Ⅰ and Hind Ⅲ, and ligated into pEGFP-N1-P7.5, yielding the recombinant vector pEGFP-N1-P7.5-H; next the expression cassette EGFP-N1-P7.5-H was first released from recombinant vector pEGFP-N1-P7.5-H by double digestion of Hind Ⅲ and Nhe Ⅰ and ligated into pUC119-TK that was digested by Kpn Ⅰ, yielding the transfer vector pUC119-TK-EGFP-P7.5-H. [Result] Identification and double enzyme digestion showed that the transfer vector pUC119-TK-EGFP-P7.5-H was correctly constructed. From the transfer vector transfected BHK-21 cells which infected GTPV AV41, specific fluorescence was observed at 48th h of transfection. [Conclusion] The construction of goat poxvirus live vector laid a foundation for the live vector vaccine of PPR vaccine.

分类号： S852.65

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