Deeply functional identification of TCS1 alleles provides efficient technical paths for low-caffeine breeding of tea plants

文献类型： 外文期刊

第一作者： Wang, Yi

作者： Wang, Yi;Liu, Yu-Fei;Wei, Meng-Yuan;Zhang, Chen-Yu;Chen, Jie-Dan;Yao, Ming-Zhe;Chen, Liang;Jin, Ji-Qiang;Liu, Yu-Fei

作者机构：

期刊名称：HORTICULTURE RESEARCH （ 影响因子：8.7； 五年影响因子：9.0 ）

ISSN： 2662-6810

年卷期： 2023 年 10 卷 2 期

页码：

收录情况： SCI

摘要： Caffeine is an important functional component in tea, which has the effect of excitement and nerve stimulation, but excessive intake can cause insomnia and dysphoria. Therefore, the production of tea with low-caffeine content can meet the consumption needs of certain people. Here, in addition to the previous alleles of the tea caffeine synthase (TCS1) gene, a new allele (TCS1h) from tea germplasms was identified. Results of in vitro activity analysis showed that TCS1h had both theobromine synthase (TS) and caffeine synthase (CS) activities. Site-directed mutagenesis experiments of TCS1a, TCS1c, and TCS1h demonstrated that apart from the 225th amino acid residue, the 269th amino acid also determined the CS activity. GUS histochemical analysis and dual-luciferase assay indicated the low promoter activity of TCS1e and TCS1f. In parallel, insertion and deletion mutations in large fragments of alleles and experiments of site-directed mutagenesis identified a key cis-acting element (G-box). Furthermore, it was found that the contents of purine alkaloids were related to the expression of corresponding functional genes and alleles, and the absence or presence and level of gene expression determined the content of purine alkaloids in tea plants to a certain extent. In summary, we concluded TCS1 alleles into three types with different functions and proposed a strategy to effectively enhance low-caffeine tea germplasms in breeding practices. This research provided an applicable technical avenue for accelerating the cultivation of specific low-caffeine tea plants.

分类号：

  • 相关文献
作者其他论文 更多>>
  • Extreme drought impacts have been underestimated in grasslands and shrublands globally

    作者：Smith, Melinda D.;Knapp, Alan K.;Ohlert, Timothy;Smith, Melinda D.;Knapp, Alan K.;Wilkins, Kate D.;Holdrege, Martin C.;Beard, Karen H.;Kulmatiski, Andrew;Holdrege, Martin C.;Beard, Karen H.;Kulmatiski, Andrew;Wilfahrt, Peter;Anderson, Maggie;Borer, Elizabeth T.;Isbell, Forest;Seabloom, Eric W.;Collins, Scott L.;Sala, Osvaldo E.;Jordan, Samuel;Dukes, Jeffrey S.;Phillips, Richard P.;Beier, Claus;Yahdjian, Laura;Biancari, Lucio;Bondaruk, Viviana Florencia;Onatibia, Gaston R.;Pineiro-Guerra, Juan Manuel;Gherardi, Laureano A.;Larsen, Klaus Steenberg;Fraser, Lauchlan H.;Jentsch, Anke;Niu, Yujie;Loik, Michael E.;Maestre, Fernando T.;Valdecantos, Alejandro;Maestre, Fernando T.;Gozalo, Beatriz;Maturano-Ruiz, Adrian;Valdecantos, Alejandro;Power, Sally A.;Chieppa, Jeff;Nielsen, Uffe N.;Yu, Qiang;Felton, Andrew J.;Munson, Seth M.;Luo, Yiqi;Bauerle, Taryn;Abdoli, Hamed;Abedi, Mehdi;Bahalkeh, Khadijeh;Alados, Concepcion L.;Castillo-Garcia, Miguel;Pueyo, Yolanda;Alberti, Juan;Bruschetti, Carlos Martin;Cardoni, Augusto;Daleo, Pedro;Iribarne, Oscar;Alon, Moshe;Cohen, Ofer;Sternberg, Marcelo;An, Hui;Anacker, Brian;Auge, Harald;Auge, Harald;Eisenhauer, Nico;Hoss, Daniela;Roscher, Christiane;Sunnemann, Marie;Bachle, Seton;Bachle, Seton;Bahn, Michael;Ingrisch, Johannes;Batbaatar, Amgaa;Cahill, James F., Jr.;Batbaatar, Amgaa;Bork, Edward W.;Carlyle, Cameron N.;Behn, Kai;Beil, Ilka;Kreyling, Juergen;Malyshev, Andrey V.;Blindow, Irmgard;Dahlke, Sven;Byrne, Kerry M.;Calvo, Dianela A.;Leder, Cintia V.;Peter, Guadalupe;Carbognani, Michele;Forte, T'ai G. W.;Petraglia, Alessandro;Chang, Scott X.;Cianciaruso, Marcus V.;Guidoni-Martins, Karlo G.;Nobre, Paola;Cordeiro, Amanda L.;Cusack, Daniela F.;Dietterich, Lee H.;D'Antonio, Carla M.;Dietterich, Lee H.;Doherty, Tim S.;Greenville, Aaron C.;Wardle, Glenda M.;Dubbert, Maren;Ebeling, Anne;Eisenhauer, Nico;Fischer, Felicia M.;Hoss, Daniela;Sunnemann, Marie;Hoss, Daniela;Pillar, Valerio D.;Fischer, Felicia M.;Gebauer, Tobias;Anacker, Brian;Haugum, Siri Vatso;Vandvik, Vigdis;Hautier, Yann;Hefting, Mariet;Henry, Hugh A. L.;Johnson, Yari;Kelly, Eugene F.;Kimmel, Kaitlin;Kroel-Dulay, Gyorgy;Orban, Ildiko;Kroel-Dulay, Gyorgy;Orban, Ildiko;Kubert, Angelika;Werner, Christiane;Lamb, Eric G.;Larson, Julie;O'Connor, Rory C.;Lawson, Jason;Plowes, Robert M.;Linstadter, Anja;Orban, Ildiko;Liu, Jielin;Liu, Shirong;Longo, Grisel;Loydi, Alejandro;Rodriguez, Dana Aylen;Scarfo, Maria Cecilia;Luan, Junwei;Wang, Yi;Luan, Junwei;Wang, Yi;Lubbe, Frederick Curtis;Wiehl, Georg;Mackie-Haas, Kathleen;Stampfi, Andreas;Merchant, Thomas;Metcalfe, Daniel B.;Mori, Akira S.;Mori, Akira S.;Mudongo, Edwin;Newman, Gregory S.;Souza, Lara;Nimmo, Dale;Ogaya, Roma;Penuelas, Josep;Osborne, Brooke;Otfinowski, Rafael;Partel, Meelis;Pineiro-Guerra, Juan Manuel;Peri, Pablo L.;Picon-Cochard, Catherine;Pineiro-Guerra, Juan Manuel;Ploughe, Laura W.;Portales-Reyes, Cristy;Reed, Sasha C.;Ritchie, Euan G.;Roscher, Christiane;Sanchez, Ana M.;Santo, Braulio A.;Shi, Baoku;Sun, Wei;Souza, Lara;Standish, Rachel J.;Zeiter, Michaela;Stampfi, Andreas;Zeiter, Michaela;Standish, Rachel J.;Tedder, Michelle;Thorvaldsen, Pal;Tian, Dashuan;Zhu, Juntao;Zong, Ning;Tielborger, Katja;van den Brink, Liesbeth;Vankoughnett, Mathew R.;Velle, Liv Guri;Wang, Changhui;Wei, Cunzheng;Williams, Jennifer L.;Wolf, Amelia A.;Zhang, Fawei;Zuo, Xiaoan;Zuo, Xiaoan;Gebauer, Tobias;Ploughe, Laura W.

    关键词：climate extreme; Drought-Net; International Drought Experiment; productivity

  • SfREPAT38, a pathogen response gene (REPAT), is involved in immune response of Spodoptera frugiperda larvae through mediating Toll signalling pathway

    作者：Wang, Yuxue;Mbiza, Natasha Isabel Tanatsiwa;Liu, Ting;Wang, Yi;Zhang, Yi;Luo, Xincheng;Chu, Longyan;Li, Jianping;Yang, Yazhen;Wang, Xiangping;Zhang, Jianmin;Yu, Yonghao

    关键词：Bacillus thuringiensis; RNA interference; SfREPAT38; Spodoptera frugiperda; Toll signalling pathway

  • A nonsynonymous mutation in BhLS, encoding an acyl-CoA N-acyltransferase leads to fruit and seed size variation in wax gourd (Benincasa hispida)

    作者：Yan, Jinqiang;Xie, Dasen;Wang, Yi;Wang, Min;Yang, Songguang;Jiang, Biao;Chen, Lin;Cai, Jinsen;Liu, Wenrui;Cheng, Jiaowen

    关键词：

  • Unveiling the driving role of pH on community stability and function during lignocellulose degradation in paddy soil

    作者：Wang, Yi;Chen, Yonglun;Gao, Xiuqing;Wang, Qiong;Cui, Mingyu;Guo, Peng;Chen, Yonglun;Gao, Xiuqing;Cui, Mingyu;Zhang, Dongdong;Guo, Peng

    关键词：paddy soil; straw incorporation; bioaugmentation; biostimulation; microbial community; pH influence

  • Disrupted Microbiota of Colon Results in Worse Immunity and Metabolism in Low-Birth-Weight Jinhua Newborn Piglets

    作者：Li, Jiaheng;Lou, Fangfang;Zhang, Xiaojun;Hu, Xujin;Tu, Pingguang;Du, Xizhong;Li, Jiaheng;Wei, Zeou;Duan, Jiujun;Luo, Chengzeng;Liu, Lei;Zhong, Ruqing;Chen, Liang;Zhang, Hongfu;Li, Jiaheng;Wei, Zeou

    关键词：Jinhua newborn piglet; low-birth-weight; microbiome; transcriptome; glucose metabolism; immunity; short chain fatty acids

  • Effects of the compound extracts of Caprifoliaceae and Scutellaria baicalensis Georgi on the intestinal microbiota and antioxidant function

    作者：Zhang, Shunfen;Tang, Shanlong;Zhong, Ruqing;Chen, Liang;Zhang, Hongfu;Zhang, Shunfen;Lv, Huiyuan;Lv, Huiyuan;Cai, Xueying

    关键词：Scutellaria baicalensis Georgi; Caprifoliaceae; gut microbe; antioxidant; inflammatory

  • Genome-wide identification of class III peroxidases in colored calla lily and enhanced resistance to soft rot bacteria

    作者：Wang, Di;Wang, Yi;Gou, Rongxin;Jiang, Yin;Zeng, Zhen;Wei, Zunzheng;Wang, Di;Gou, Rongxin;Jiang, Yin;Zhang, Guojun;Yang, Tuo

    关键词：Class III peroxidases; Soft rot; MeJA; BTH

微信小程序