Regulation of seed oil accumulation by lncRNAs in Brassica napus

文献类型： 外文期刊

第一作者： Li, Yuqing

作者： Li, Yuqing;Tan, Zengdong;Zeng, Chenghao;Xiao, Mengying;Lin, Shengli;Yao, Wei;Li, Qing;Guo, Liang;Lu, Shaoping;Li, Yuqing;Tan, Zengdong;Zeng, Chenghao;Xiao, Mengying;Lin, Shengli;Yao, Wei;Li, Qing;Guo, Liang;Lu, Shaoping;Guo, Liang;Guo, Liang

作者机构：

关键词： LncRNA; Oil content; Lipid; Metabolite; Transgenic plants; Brassica napus

期刊名称：BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS

ISSN：

年卷期： 2023 年 16 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundStudies have indicated that long non-coding RNAs (lncRNAs) play important regulatory roles in many biological processes. However, the regulation of seed oil biosynthesis by lncRNAs remains largely unknown.ResultsWe comprehensively identified and characterized the lncRNAs from seeds in three developing stages in two accessions of Brassica napus (B. napus), ZS11 (high oil content) and WH5557 (low oil content). Finally, 8094 expressed lncRNAs were identified. LncRNAs MSTRG.22563 and MSTRG.86004 were predicted to be related to seed oil accumulation. Experimental results show that the seed oil content is decreased by 3.1-3.9% in MSTRG.22563 overexpression plants, while increased about 2% in MSTRG.86004, compared to WT. Further study showed that most genes related to lipid metabolism had much lower expression, and the content of some metabolites in the processes of respiration and TCA (tricarboxylic acid) cycle was reduced in MSTRG.22563 transgenic seeds. The expression of genes involved in fatty acid synthesis and seed embryonic development (e.g., LEC1) was increased, but genes related to TAG assembly was decreased in MSTRG.86004 transgenic seeds.ConclusionOur results suggest that MSTRG.22563 might impact seed oil content by affecting the respiration and TCA cycle, while MSTRG.86004 plays a role in prolonging the seed developmental time to increase seed oil accumulation.

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