Construction of chimeric viruses based on pepper mild mottle virus using a modified Cre/loxP system

文献类型： 外文期刊

第一作者： Yin, Yue-yan

作者： Yin, Yue-yan;Wan, Qiong-lian;Chen, Hai-ru;Chen, Jian-ping;Yin, Yue-yan;Hua, Meng-ying;Zhao, Kuang-jie;Lu, Yu-wen;Zheng, Hong-ying;Rao, Shao-fei;Yan, Fei;Peng, Jie-jun;Chen, Jian-ping;Bu, Shan

作者机构：

关键词： pepper mild mottle virus; Cre; loxP; necrosis; infectious cDNA clone; chimeric virus

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：4.384； 五年影响因子：4.021 ）

ISSN： 2095-3119

年卷期： 2022 年 21 卷 8 期

页码：

收录情况： SCI

摘要： Cre/loxP, a site-specific recombination system, has been widely used for various purposes, including chromosomal translocations, generation of marker-free transgenic plants, tissue-specific activation of a reporter gene and efficient heterologous gene expression in plants. However, stable or transient expression of Cre recombinase in plants can cause chlorosis or necrosis. Here, we describe a modified Cre/loxP recombination system using a DNA fragment flanked with loxP sites in the same orientation in which necrosis induced by Cre recombinase in Nicotiana benthamiana leaves was alleviated. The modified system was successfully used to create functional GFP-tagged pepper mild mottle virus (PMMoV) and a chimeric virus with coat protein (CP) substitution assembled from separate pro-vector modules. Our results provide a new strategy and flexible technique to construct chimeric virus and infectious clones for plant viruses with large genomes.

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