CsTPS21 encodes a jasmonate-responsive monoterpene synthase producing beta-ocimene in citrus against Asian citrus psyllid

文献类型： 外文期刊

第一作者： Bin, Minliang

作者： Bin, Minliang;Yi, Ganjun;Zhang, Xinxin;Bin, Minliang;Peng, Xinxiang;Zhang, Xinxin

作者机构：

关键词： Asian citrus psyllid; Citrus sinensis; CsTPS21; beta-ocimene; Herbivore defense; JA pathway

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：6.5； 五年影响因子：6.4 ）

ISSN： 0981-9428

年卷期： 2023 年 201 卷

页码：

收录情况： SCI

摘要： Huanglongbing (HLB), spread by the Asian citrus psyllid (ACP), is a widespread, devastating disease that causes significant losses in citrus production. Therefore, controlling the ACP infestation and HLB infection is very important for citrus production. The aim of our study was to identify any citrus volatile which could be used as a repellent or less attractant towards ACP, and to envisage the potential of this strategy to control HLB spread. The present study identified a terpene synthase (TPS)-encoding gene CsTPS21 in citrus plants, and this gene was predicted to encode a monoterpene synthase and had an amino acid sequence similar to beta-ocimene synthase. CsTPS21 was significantly upregulated by ACP infestation and methyl jasmonic acid (MeJA) treatment but downregulated by salicylic acid (SA). Further heterologous gene expression studies in yeast cells and tobacco plants indicated that the protein catalyzed the formation of beta-ocimene, which acted as an ACP repellent. Detailed analysis of tobacco overexpressing CsTPS21 showed that CsTPS21 synthesizing beta-ocimene regulated jasmonic acid (JA)-associated pathways by increasing the JA accumulation and inducing the JA biosynthetic gene expression to defend against insect infestation. These findings provide a basis to plan strategies to manage HLB in the field using beta-ocimene and CsTPS21 as candidates.

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