A standardized detection method for various poultry egg yolk immunoglobulin based on effective sample pretreatment and size exclusion-HPLC

文献类型： 外文期刊

第一作者： Zhu, Yan

作者： Zhu, Yan;Zhang, Xinyue;Huang, Xi;Cai, Zhaoxia;Wu, Wenjuan;Tan, Lei;Qiu, Yongwei;Luo, Yan;Zeng, Tao;Lu, Lizhi;Hu, Wei

作者机构：

关键词： Yolk immunoglobulin; SE-HPLC; Poultry egg yolk; Water dilution separation; Standardized detection method

期刊名称：JOURNAL OF FOOD COMPOSITION AND ANALYSIS （ 影响因子：4.6； 五年影响因子：4.6 ）

ISSN： 0889-1575

年卷期： 2025 年 140 卷

页码：

收录情况： SCI

摘要： Currently there are extremely few detailed reports on the quantitative detection of egg yolk immunoglobulin (IgY). Moreover, there is still a gap in standardized methods for IgY detection in poultry eggs. Size exclusion (SE) high performance liquid chromatography (HPLC) is a separation technique based on molecular size difference, and can be used as a standard method. Therefore, this study established a simple quantitative analysis method for IgY in various poultry eggs, based on the water dilution IgY extraction pretreatment, combined with SE-HPLC. The method was optimized for pretreatment and SE-HPLC detection conditions for five poultry eggs, and the analytical performance was evaluated by baseline separation. The results showed that poultry egg yolks diluted 10-fold with water and further purified by hexane could be used for SE-HPLC analysis. The optimized detection conditions for SE-HPLC were AdvanceBio SEC 300 A for the column, phosphate buffer containing 0.1 mol/L Na2SO4 (80 % mobile phase A) and acetonitrile (20 % mobile phase B) for the mobile phase at a flow rate of 0.8 mL/min, and a detection wavelength of 280 nm. The HPLC method showed good linearity (R-2 > 99 %), RSD < 10 %, satisfactory spiked recoveries (75.0 % similar to 105.3 %) and good reproducibility.

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