Rapid Sample Enrichment, Novel Derivatization, and High Sensitivity for Determination of 3-Chloropropane-1,2-diol in Soy Sauce via High-Performance Liquid Chromatography-Tandem Mass Spectrometry

文献类型： 外文期刊

第一作者： Tang, Yunyu

作者： Tang, Yunyu;Yang, Guangxin;Shen, Xiaosheng;Wang, Yuan;Lou, Xiaoyi;Kong, Cong;Liu, Xiaoxia;Qin, Lixia;Zhai, Wenlei;Fodjo, Essy Kouadio

作者机构：

关键词： 3-MCPD; HPLC-MS/MS; acid-hydrolyzed vegetableprotein; food contaminant; facile derivatization; carbon yarn; p-(dimethylamino)-phenol

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.1； 五年影响因子：6.3 ）

ISSN： 0021-8561

年卷期： 2023 年 71 卷 41 期

页码：

收录情况： SCI

摘要： A novel, simplified derivatization method and a rapid sample preparation process using carbon yarn as a sorbent for the determination of 3-chloropropane-1,2-diol (3-MCPD) in soy sauce via high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was developed. 3-MCPD was first enriched and purified with carbon yarn and then eluted with a methanol-water solution. Subsequently, the analyte underwent derivatization with p-(dimethylamino)-phenol for sensitive detection via HPLC-MS/MS. The limit of detection and the limit of quantitation for 3-MCPD were validated to be 0.5 and 1.0 mu g/kg, respectively. Spiking experiments showed recoveries between 83 and 94%, with a relative standard deviation of <= 10%. The method was further validated with a certified reference material. Furthermore, 11 real soy sauce samples from local markets were tested by using this method. These results reveal the widespread 3-MCPD contamination. Consequently, this study offers a preferable alternative for the sensitive, accurate, and precise determination of 3-MCPD in soy sauce.

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