Molecular characterization and pathogenicity of an infectious cDNA clone of tomato brown rugose fruit virus

文献类型： 外文期刊

第一作者： Ma, Ziyue

作者： Ma, Ziyue;Yang, Xiuling;Zhou, Xueping;Zhang, Hui;Ding, Ming;Zhang, Zhongkai;Zhou, Xueping

作者机构：

关键词： Tobamovirus; Tomato brown rugose fruit virus; Infectious clone; Agroinoculation

期刊名称：PHYTOPATHOLOGY RESEARCH （ 影响因子：3.955； 五年影响因子：3.955 ）

ISSN： 2096-5362

年卷期： 2021 年 3 卷 1 期

页码：

收录情况： SCI

摘要： Tomato brown rugose fruit virus (ToBRFV) is a new member of the genus Tobamovirus, and has the potential to affect the production and marketability of tomatoes and peppers. In this study, we sequenced and analyzed the complete genome of ToBRFV isolates from tomato plants showing mosaic and mottling symptoms in Yunnan Province of China. We constructed a full-length infectious cDNA clone of ToBRFV, which could induce systemic infection with typical symptoms in tomato, Nicotiana benthamiana, and N. tabacum cv. Samsun nn plants through Agrobacterium-mediated inoculation. Further experimental evidence demonstrated that the rod-shaped virions accumulating in agroinfiltrated plants are sap-transmissible. This is the first report on the construction of a biologically active, full-length infectious cDNA clone of ToBRFV. The system developed herein will facilitate further research on functions of ToBRFV-encoded proteins and plant-ToBRFV interactions through reverse genetic approaches.

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[1]Molecular characterization and pathogenicity of an infectious cDNA clone of tomato brown rugose fruit virus. Ma, Ziyue,Yang, Xiuling,Zhou, Xueping,Zhang, Hui,Ding, Ming,Zhang, Zhongkai,Zhou, Xueping. 2021

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