Citrate accumulation mediated through GhCS6 enhances antioxidant modulation under Cd2+stress in cotton

文献类型： 外文期刊

第一作者： Zhang, Xinrui

作者： Zhang, Xinrui;Lu, Xuke;Fan, Yapeng;Yang, Zhining;Sun, Yuping;Yu, Xin;Song, Ruize;Zhang, Menghao;Lan, Hao;Chen, Xiugui;Wang, Shuai;Wang, Junjuan;Guo, Lixue;Li, Ying;Zhao, Lanjie;Ye, Wuwei;Zhang, Xinrui;Hao, Fushun;Dai, Maohua;Li, Lin

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关键词： Citrate; Citrate synthase; GhCSs; Cd 2+stress; ROS

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：5.7； 五年影响因子：6.4 ）

ISSN： 0981-9428

年卷期： 2025 年 228 卷

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收录情况： SCI

摘要： Citrate synthase (CS) catalyzes the biosynthesis of citrate (CA), a tricarboxylic acid metabolite that serves as a pivotal biochemical regulator in plant cellular metabolism. This investigation conducted systematic genomewide identification of CS family, integrating structural, phylogenetic relationships, collinearity analysis, cis-acting elements and expression analysis. GhCS6 protein is localized in the mitochondria. The pivotal cadmium-responsive regulatory candidate gene GhCS6 was identified, followed by its silencing mediation through virus-induced gene silencing (VIGS), silenced plants displaying phenotypic evidence of enhanced Cd2+ susceptibility. Simultaneously, CA content in the silenced plants was also significantly decreased, which reduced the chelating capacity for Cd2+ and consequently exacerbates the accumulation of Cd2+. The silenced plants showed an imbalance in the reactive oxygen species (ROS) scavenging system, as evidenced by lower superoxide dismutase (SOD) activity and a marked increase in H2O2 content, causing increased oxidative damage. This oxidative stress further compromised the integrity of cellular membranes, resulting in elevated malondialdehyde (MDA) content and compromised cellular structure. These changes ultimately impaired photosynthetic efficiency and plant growth and development. This study contributes significantly to understanding the function of the GhCS6 in plant Cd2+ tolerance, and identifies potential gene targets for breeding Cd2+ tolerant cotton varieties.

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