Cloning and Characterization of a CAP Gene Expressed in Gossypium arboreum Fuzzless Mutant

文献类型： 外文期刊

第一作者： Sheng Wang

作者： Sheng Wang;Guo-Hong Zhao;Yin-Hua Jia;Xiong-Ming Du

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关键词： signal transduction;cytoskeletal organization;fiber development;polar substitution

期刊名称：CROP SCIENCE （ 影响因子：2.319； 五年影响因子：2.631 ）

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收录情况： SCI

摘要： The adenylyl cyclase-associated protein (CAP) was first identified in the budding yeast as a protein that functions in cytoskeletal organization and signal transduction. We isolated two CAP homologs from wild-type cotton Gossypium arboreum L. (DPL971) and its natural fuzzless mutant (DPL972). Alignments of 3998-bp genomic and 1425-bp cDNA sequences revealed that the cap genes contain nine introns and an open reading frame (ORF) encoding a 471 amino acid protein. The predicted amino acid sequence shares high identity (similar to 70%) with Arabidopsis thaliana, Medicago truncatula, and Oryza sativa. Expression profiles of the gene were studied from ovule, leaf, hypocotyl, and root tissues, through reverse transcriptase polymerase chain reaction and Northern blotting analysis. The results revealed that the gene was expressed in all tissues tested in wild-type and fuzzless plants. The expression of GaCAP reached the highest level in both wild-type and mutant fibers during 1 to 4 d postanthesis (DPA), then gradually declined in the mutant after 4 DPA. By comparing the predicted amino acid sequences, a polar substitution was observed at a conserved position between wild-type and mutant. At this position, the conserved uncharged unpolar alanine (amino acid 44) of GaCAP was replaced by a polar threonine in GaCAPm. Therefore, our results suggest that GaCAP may play a functional role during early stages of cotton fiber development.

分类号： S5

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