Heat shock protein 70 and Cathepsin B genes are involved in the thermal tolerance of Aphis gossypii

文献类型： 外文期刊

第一作者： Liu, Jinping

作者： Liu, Jinping;Liu, Yang;Li, Qian;Lu, Yanhui;Lu, Yanhui

作者机构：

关键词： Aphis gossypii; RNA interference; Heat shock protein; Differentially expressed gene; RNA-seq

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：4.1； 五年影响因子：4.4 ）

ISSN： 1526-498X

年卷期： 2023 年 79 卷 6 期

页码：

收录情况： SCI

摘要： BACKGROUNDElevated temperature can directly affect the insect pest population dynamics. Many experimental studies have indicated that high temperatures affect the biological and ecological characteristics of the widely distributed crop pest Aphis gossypii, but the molecular mechanisms underlying its response to heat stress remain unstudied. Here, we used transcriptomic analysis to explore the key genes and metabolic pathways involved in the regulation of thermotolerance in A. gossypii at 29 degrees C, 32 degrees C, and 35 degrees C. RESULTSThe results of bioinformatics analysis show that few genes were consistently differentially expressed among the higher temperature treatments compared to 29 degrees C, and a moderate temperature increase of 3 degrees C can elicit gene expression changes that help A. gossypii adapt to warmer temperatures. Based on KEGG pathway enrichment analysis, we found that genes encoding four heat shock protein 70 s (Hsp70s) and nine cathepsin B (CathB) proteins were significantly upregulated at 35 degrees C compared with 32 degrees C. Genes related to glutathione production were also highly enriched between 32 degrees C and 29 degrees C. Silencing of two Hsp70s (ApHsp70A1-1 and ApHsp68) and two CathBs (ApCathB01 and ApCathB02) with RNA interference using a nanocarrier-based transdermal dsRNA delivery system significantly increased sensitivity of A. gossypii to high temperatures. CONCLUSIONA. gossypii is able to fine-tune its response across a range of temperatures, and Hsp70 and CathB genes are essential for adaption of A. gossypii to warmer temperatures. (c) 2023 Society of Chemical Industry.

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