Isolation and identification of phenolic compounds in Chinese purple yam and evaluation of antioxidant activity
文献类型: 外文期刊
第一作者: Zhang, Junhong
作者: Zhang, Junhong;Du, Fangling;Zong, Aizhen;Xu, Tongcheng;Zhang, Junhong;Tian, Honglei;Zhan, Ping
作者机构:
关键词: Purple yam; HPLC-UV; ESI-MS; Phenolic compounds; Caffeic acid
期刊名称:LWT-FOOD SCIENCE AND TECHNOLOGY ( 影响因子:4.952; 五年影响因子:5.383 )
ISSN: 0023-6438
年卷期: 2018 年 96 卷
页码:
收录情况: SCI
摘要: Purple yam (Dioscorea alata L) has been extensively investigated in the fields of biomedicine, pharmacology, and toxicology. As functional components, phenolic compounds exhibit high levels of biological activities. In this study, seven phenolic compounds extracted from Chinese purple yam were successfully identified using highperformance liquid chromatography combined with high-resolution electrospray ionization mass spectrometry. Comparison of the retention time and mass spectra of the extracted compounds with those of authentic standards confirmed the existence of ferulic acid (4) and sinapic acid (5). And five kinds of the previously unidentified phenolic compounds were revealed, including one kind of dihydroxybenzoic acid (vanillic acid, 2), two sets of hydroxycinnamic acids (caffeic acid, 1 and p-coumaric acid, 3), and two kinds of anthocyanins (quercetin dehydrate, 6 and kaempferol, 7). In addition, the total antioxidant activity and DPPH free radical scavenging activity of the isolated fractions were evaluated, and the concentrated F-1 fraction displayed a high total antioxidant activity (2.38 mM) and DPPH free radical scavenging activity (89.56%). This study provided an in-depth insight into the diversified components and antioxidant activity of purple yam. Such information is beneficial to the post processing and storage of purple yam products.
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