Matrix-glycoprotein interactions required for budding of a plant nucleorhabdovirus and induction of inner nuclear membrane invagination

文献类型： 外文期刊

第一作者： Sun, Kai

作者： Sun, Kai;Zhou, Xin;Lin, Wenye;Zhou, Xueping;Li, Zhenghe;Zhou, Xueping;Jackson, Andrew O.

作者机构：

关键词： budding;glycoprotein;matrix protein;nuclear membrane invagination;nucleorhabdovirus;plant rhabdovirus;sonchus yellow net virus

期刊名称：MOLECULAR PLANT PATHOLOGY （ 影响因子：5.663； 五年影响因子：5.626 ）

ISSN： 1464-6722

年卷期： 2018 年 19 卷 10 期

页码：

收录情况： SCI

摘要： Nucleorhabdoviruses such as Sonchus yellow net virus (SYNV) replicate in the nuclei and undergo morphogenesis at the inner nuclear membrane (IM) in plant cells. Mature particles are presumed to form by budding of the Matrix (M) protein-nucleocapsid complexes through host IMs to acquire host phospholipids and the surface glycoproteins (G). To address mechanisms underlying nucleorhabdovirus budding, we generated recombinant SYNV G mutants containing a truncated amino-terminal (NT) or carboxyl-terminal (CT) domain. Electron microscopy and sucrose gradient centrifugation analyses showed that the CT domain is essential for virion morphogenesis whereas the NT domain is also required for efficient budding. SYNV infection induces IM invaginations that are thought to provide membrane sites for virus budding. We found that in the context of viral infections, interactions of the M protein with the CT domain of the membrane-anchored G protein mediate M protein translocation and IM invagination. Interestingly, tethering the M protein to endomembranes, either by co-expression with a transmembrane G protein CT domain or by artificial fusion with the G protein membrane targeting sequence, induces IM invagination in uninfected cells. Further evidence to support functions of G-M interactions in virus budding came from dominant negative effects on SYNV-induced IM invagination and viral infections that were elicited by expression of a soluble version of the G protein CT domain. Based on these data, we propose that cooperative G-M interactions promote efficient SYNV budding.

分类号： Plant Sciences

  • 相关文献

[1]The gene 4 of rice yellow stunt rhabdovirus encodes the matrix protein. Luo, ZL,Chen, XY,Gao, DM,Fang, RX. 1998

[2]Identification of CD8(+) cytotoxic T lymphocyte epitopes from porcine reproductive and respiratory syndrome virus matrix protein in BALB/c mice. Zhang, Weijun,Lin, Yan,Bai, Yu,Tong, Tiegang,Wang, Qun,Liu, Nihong,Liu, Guangliang,Xiao, Yihong,Yang, Tao,Bu, Zhigao,Wu, Donglai,Tong, Guangzhi,Zhang, Weijun,Lin, Yan,Bai, Yu,Tong, Tiegang,Wang, Qun,Liu, Nihong,Liu, Guangliang,Xiao, Yihong,Yang, Tao,Bu, Zhigao,Tong, Guangzhi,Wu, Donglai. 2011

[3]Purification and partial characterization of a novel hemagglutinating glycoprotein from the cultured mycelia of Hericium erinaceus. Cui, Feng-Jie,Li, Yun-Hong,Zan, Xin-Yi,Sun, Wen-Jing,Qian, Jing-Ya,Yang, Yan,Cui, Feng-Jie,Sun, Wen-Jing,Zhou, Qian,Yu, Si-Lian. 2014

[4]Recombinant lentogenic Newcastle disease virus expressing Ebola virus GP infects cells independently of exogenous trypsin and uses macropinocytosis as the major pathway for cell entry. Wen, Zhiyuan,Zhao, Bolin,Song, Kun,Hu, Xule,Chen, Weiye,Kong, Dongni,Ge, Jinying,Bu, Zhigao. 2013

[5]Mechanistic understanding of N-glycosylation in Ebola virus glycoprotein maturation and function. Wang, Bin,Wang, Yujie,Yao, Xiaoyu,Hu, Dan,Zhang, Zhuo,Zheng, Yong-Hui,Liu, Chaonan,Zheng, Shimin,Xiang, Shi-Hua,Xiang, Shi-Hua,Zheng, Yong-Hui.

[6]Newcastle disease virus-vectored Nipah encephalitis vaccines induce B and T cell responses in mice and long-lasting neutralizing antibodies in pigs. Kong, Dongni,Wen, Zhiyuan,Su, Hua,Ge, Jinying,Chen, Weiye,Wang, Xijun,Wu, Chao,Chen, Hualan,Bu, Zhigao,Kong, Dongni,Wen, Zhiyuan,Su, Hua,Ge, Jinying,Chen, Weiye,Wang, Xijun,Wu, Chao,Chen, Hualan,Bu, Zhigao,Kong, Dongni,Yang, Chinglai.

[7]HIV-1 Vpr increases Env expression by preventing Env from endoplasmic reticulum-associated protein degradation (ERAD). Zhang, Xianfeng,Zhou, Tao,Zheng, Yong-Hui,Zhang, Xianfeng,Zhou, Tao,Frabutt, Dylan A.,Zheng, Yong-Hui.

[8]Purification and partial characterization of a novel anti-tumor glycoprotein from cultured mycelia of Grifola frondosa. Cui, Fengjie,Zan, Xinyi,Li, Yunhong,Sun, Wenjing,Dong, Ying,Cui, Fengjie,Sun, Wenjing,Zhou, Qiang,Yu, Silian,Yang, Yan,Sun, Wenjing,Zhou, Qiang,Yu, Silian.

[9]Chicken galectin-1B inhibits Newcastle disease virus adsorption and replication through binding to hemagglutinin-neuraminidase (HN) glycoprotein. Sun, Junfeng,Han, Zongxi,Qi, Tianming,Zhao, Ran,Liu, Shengwang. 2017

[10]Protective efficacy of a recombinant Newcastle disease virus expressing glycoprotein of vesicular stomatitis virus in mice. Zhang, Minmin,Ge, Jinying,Li, Xiaofang,Chen, Weiye,Wang, Xijun,Wen, Zhiyuan,Bu, Zhigao. 2016

[11]Optimization of Fermentation Conditions and Bench-Scale for Improvement of a Novel Glycoprotein GP-1 Production by Streptomyces kanasenisi ZX01. Zhou, Yong,Zhou, Xin,Feng, Jun-Tao,Han, Li-Rong,Zhang, Xing,Yu, Dai-Lin,Sang, Bu,Feng, Jun-Tao,Han, Li-Rong,Zhang, Xing. 2018

[12]Preparation of Molecularly Imprinted Polymers Functionalized with Core-shell Magnetic Nanoparticles for the Recognition of Glycoprotein. Wang, He-Ye,Li, Li,Zhao, Yong-Fu. 2015

[13]Effects of Agitation, Aeration and Temperature on Production of a Novel Glycoprotein GP-1 by Streptomyces kanasenisi ZX01 and Scale-Up Based on Volumetric Oxygen Transfer Coefficient. Zhou, Yong,Han, Li-Rong,He, Hong-Wei,Feng, Jun-Tao,Zhang, Xing,Han, Li-Rong,Feng, Jun-Tao,Zhang, Xing,Sang, Bu,Yu, Dai-Lin. 2018

[14]High throughput screening of recombinant antibodies against infectious hematopoietic necrosis virus from a combinatorial antibody library. Xu, Li-Ming,Zhao, Jing-Zhuang,Liu, Miao,Cao, Yong-Sheng,Yin, Jia-Sheng,Liu, Hong-Bai,Tongyan-Lu.

作者其他论文 更多>>

微信小程序