Regulation of Nicotine Biosynthesis by an Endogenous Target Mimicry of MicroRNA in Tobacco

文献类型： 外文期刊

第一作者： Li, Fangfang

作者： Li, Fangfang;Wang, Weidi;Zhao, Nan;Ye, Chuyu;Shen, Enhui;Qiu, Jie;Fan, Longjiang;Li, Fangfang;Wang, Weidi;Zhao, Nan;Ye, Chuyu;Shen, Enhui;Qiu, Jie;Fan, Longjiang;Li, Fangfang;Zhao, Nan;Zhou, Xueping;Xiao, Bingguang;Wu, Xingfu;Cao, Peijian;Zhu, Qian-Hao;Xie, Jiahua

作者机构：

期刊名称：PLANT PHYSIOLOGY （ 影响因子：8.34； 五年影响因子：8.972 ）

ISSN： 0032-0889

年卷期： 2015 年 169 卷 2 期

页码：

收录情况： SCI

摘要： The interaction between noncoding endogenous target mimicry (eTM) and its corresponding microRNA (miRNA) is a newly discovered regulatory mechanism and plays pivotal roles in various biological processes in plants. Tobacco (Nicotiana tabacum) is a model plant for studying secondary metabolite alkaloids, of which nicotine accounts for approximately 90%. In this work, we identified four unique tobacco-specific miRNAs that were predicted to target key genes of the nicotine biosynthesis and catabolism pathways and an eTM, novel tobacco miRNA (nta)-eTMX27, for nta-miRX27 that targets QUINOLINATE PHOSPHORIBOSYLTRANSFERASE2 (QPT2) encoding a quinolinate phosphoribosyltransferase. The expression level of nta-miRX27 was significantly down-regulated, while that of QPT2 and nta-eTMX27 was significantly up-regulated after topping, and consequently, nicotine content increased in the topping-treated plants. The topping-induced down-regulation of nta-miRX27 and up-regulation of QPT2 were only observed in plants with a functional nta-eTMX27 but not in transgenic plants containing an RNA interference construct targeting nta-eTMX27. Our results demonstrated that enhanced nicotine biosynthesis in the topping-treated tobacco plants is achieved by nta-eTMX27-mediated inhibition of the expression and functions of nta-miRX27. To our knowledge, this is the first report about regulation of secondary metabolite biosynthesis by an miRNA-eTM regulatory module in plants.

分类号： Plant Sciences

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