Identification of an RNA silencing suppressor encoded by a mastrevirus

文献类型： 外文期刊

第一作者： Wang, Yaqin

作者： Wang, Yaqin;Dang, Mingqing;Hou, Huwei;Mei, Yuzhen;Qian, Yajuan;Zhou, Xueping

作者机构：

期刊名称：JOURNAL OF GENERAL VIROLOGY （ 影响因子：3.891； 五年影响因子：3.719 ）

ISSN： 0022-1317

年卷期： 2014 年 95 卷

页码：

收录情况： SCI

摘要： Wheat dwarf virus (WDV) is a DNA virus belonging to the genus Mastrevirus of the family Geminiviridae. In this study, we report that the Rep protein encoded by WDV is a RNA silencing supressor as determined by co-infiltration assays using transgenic Nicotiana benthamiana line 16c carrying the GFP reporter gene. The Rep protein was shown to inhibit both local and systemic RNA silencing of the GFP gene as well as the spread of systemic GFP RNA silencing signals. Gel mobility shift assays showed that the Rep protein binds 21 nt and 24 nt small interfering RNA (siRNA) duplexes and single-stranded (ss)-siRNA. To our knowledge, this is the first identification of an RNA silencing suppressor encoded by mastreviruses. Furthermore, deletion mutagenesis indicates that both the N- and C-terminal regions of the Rep protein are not critical for silencing suppression and self-interaction, but the N terminus of Rep is necessary for its pathogenicity.

分类号： Biotechnology & Applied Microbiology; Virology

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