V2 of tomato yellow leaf curl virus can suppress methylation-mediated transcriptional gene silencing in plants

文献类型： 外文期刊

第一作者： Wang, Bi

作者： Wang, Bi;Li, Fangfang;Huang, Changjun;Yang, Xiuling;Qian, Yajuan;Xie, Yan;Zhou, Xueping

作者机构：

期刊名称：JOURNAL OF GENERAL VIROLOGY （ 影响因子：3.891； 五年影响因子：3.719 ）

ISSN： 0022-1317

年卷期： 2014 年 95 卷

页码：

收录情况： SCI

摘要： Tomato yellow leaf curl virus (TYLCV) is a DNA virus belonging to the genus Begomovirus. TYLCV replicates using double-stranded DNA intermediates that can become the target of plant transcriptional gene silencing (TGS). Here, we show that the V2 protein of TYLCV can suppress TGS of a transcriptionally silenced green fluorescent protein (GFP) transgene in Nicotiana benthamiana line 16-TGS. Through bisulfite sequencing and chop-PCR, we demonstrated that the TYLCV V2 can reverse GFP transgene silencing by reducing the methylation levels in the 35S promoter sequence. Both AtSN1 and MEA-ISR loci in Arabidopsis thaliana were previously reported to be strongly methylated, and we show that the methylation status of both loci was significantly reduced in TYLCV V2 transgenic Arabidopsis plants. We conclude that TYLCV can efficiently suppress TGS when it infects plants, and its V2 protein is responsible for the TGS suppression activity.

分类号： Biotechnology & Applied Microbiology; Virology

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