Cytoplasmic Assembly and Selective Nuclear Import of Arabidopsis ARGONAUTE4/siRNA Complexes

文献类型： 外文期刊

第一作者： Ye, Ruiqiang

作者： Ye, Ruiqiang;Zhou, Xueping;Qi, Yijun;Ye, Ruiqiang;Wang, Wei;Liu, Chang;Wu, Yang;Qi, Yijun;Wang, Wei;Wang, Wei;Iki, Taichiro;Ishikawa, Masayuki;Liu, Chang;Qi, Yijun;Qi, Yijun

作者机构：

期刊名称：MOLECULAR CELL （ 影响因子：17.97； 五年影响因子：19.639 ）

ISSN： 1097-2765

年卷期： 2012 年 46 卷 6 期

页码：

收录情况： SCI

摘要： In plants, DNA methylation can be mediated by a class of Argonaute4 (AGO4)-associated heterochromatic siRNAs (hc-siRNAs), through a pathway termed RNA-directed DNA methylation (RdDM). It has been thought that RdDM is solely a nuclear process, as both the biogenesis and functioning of hc-siRNAs take place in the nucleus. In this study, we unexpectedly found that hc-siRNAs are predominantly present in the cytoplasm. We demonstrated that AGO4 is loaded with hc-siRNAs in the cytoplasm and the formation of mature AGO4/siRNA complexes requires HSP90 and the cleavage activity of AGO4. Intriguingly, siRNA binding facilitates the redistribution of AGO4 into the nucleus, likely through inducing conformational change that leads to the exposure of the nuclear localization signal (NLS). Our findings reveal an unsuspected cytoplasmic step in the RdDM pathway. We propose that selective nuclear import of mature AGO4/siRNA complexes is a key regulatory point prior to the effector stage of RdDM.

分类号： Biochemistry & Molecular Biology; Cell Biology

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