beta C1 encoded by tomato yellow leaf curl China betasatellite forms multimeric complexes in vitro and in vivo

文献类型： 外文期刊

第一作者： Cheng, Xiaofei

作者： Cheng, Xiaofei;Wu, Jianxiang;Zhou, Xueping;Wang, Xiaoqiang;Briddon, Rob W.

作者机构：

关键词： beta C1;TYLCCNB;Multimerization;Granular bodies;Betasatellite

期刊名称：VIROLOGY （ 影响因子：3.616； 五年影响因子：3.967 ）

ISSN： 0042-6822

年卷期： 2011 年 409 卷 2 期

页码：

收录情况： SCI

摘要： The beta C1 protein encoded by betasatellites associated with begomoviruses is multi-functional. To investigate its properties, the beta C1 protein encoded by tomato yellow leaf curl China betasatellite (TYLCCNB) was expressed in Escherichia coli and analyzed for its ability to self-interaction. The beta C1 protein formed large soluble multimeric complexes in vitro and in vivo. Mutations that prevented formation of multimeric complexes in vitro, also prevented formation of granular bodies in vivo, suggesting that granular bodies resulted from beta C1 oligomerization. Similarly, beta C1 mutants unable to form complexes also did not induce typical symptoms in plants when expressed from a Potato virus X (PVX) vector, suggesting that beta C1 self-interaction was required for symptom induction in planta. Deletion analysis revealed that amino acid sequences spanning two predicted cc-helices at the C-terminal end of the protein were important in multimerization. (C) 2010 Elsevier Inc. All rights reserved.

分类号： Virology

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