Monoclonal antibody-based triple antibody sandwich-enzyme-linked immunosorbent assay and immunocapture reverse transcription-polymerase chain reaction for Odontoglossum ringspot virus detection

文献类型： 外文期刊

第一作者： Wu, Jianxiang

作者： Wu, Jianxiang;Meng, Chunmei;Shang, Haili;Hong, Jian;Zhou, Xueping;Rong, Song;Zhang, Chao

作者机构：

关键词： Orchid;Odontoglossum ringspot virus;Monoclonal antibody;Enzyme-linked immunosorbent assay;Immunocapture RT-PCR

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）

ISSN： 0166-0934

年卷期： 2011 年 171 卷 1 期

页码：

收录情况： SCI

摘要： Odontoglossum ringspot virus (ORSV) infects numerous commercially important orchids and causes significant losses worldwide. The coat protein (CP) gene of ORSV was cloned and expressed in Escherichia coli by using the pET-32a expression vector, and the expression of recombinant protein was confirmed by Western blotting using anti-ORSV antibodies. The recombinant protein was purified using Ni-NTA agarose, and the purified protein was used as an immunogen to produce monoclonal antibodies (MAbs) and polyclonal antibodies (PAbs). Five murine MAbs against ORSV CP were obtained. Among them, two MAbs (6B4 and 1D1) also reacted with TMV CP. The triple antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA) and immunocapture reverse transcription-polymerase chain reaction (IC-RT-PCR) methods using the MAb (8A5) were then developed for sensitive, specific, and rapid detection of ORSV. TAS-ELISA and IC-RT-PCR could detect ORSV in the infected leaf saps with dilutions of 1:10,240 and 1:81,920 (w/v, g mL(-1)), respectively. TAS-ELISA and IC-RT-PCR detections indicated that ORSV was prevalent in orchids in the Zhejiang Province of China. (C) 2010 Elsevier B.V. All rights reserved.

分类号： Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Virology

  • 相关文献

[1]Development of Sensitive Monoclonal Antibody-based Enzyme-linked Immunosorbent Assay for Renal Toxicity Ingredient Aristolochic acid A in Chinese Herb Medicine. Nan Tie-Gui,He Su-Ping,Tan Gui-Yu,Wang Bao-Min,Li Gang,Huang Lu-Qi. 2010

[2]Monoclonal antibody based enzyme-linked immunosorbent assay for the specific detection of ciprofloxacin and enrofloxacin residues in fishery products. Hu, Kun,Jiang, Yousheng,Fang, Wei,Yang, Xianle,Huang, Xuanyun,Yang, Xianle.

[3]Production and Identification of Broad Spectrum Monoclonal Antibody against a Group of Pyrethroid Insecticides. Wen Meng-Tang,Liu Yuan,Yan Shuai,Zhang Xiao,Wang Heng,Liu Xian-Jin,Wen Meng-Tang,Yan Shuai. 2014

[4]A sensitive monoclonal antibody-based enzyme-linked immunosorbent assay for the detection of bifenthrin in a chemical soil barrier. Hua, Xiude,Liu, Xiaofeng,Yin, Wei,Shi, Haiyan,Liu, Fengquan,Wang, Minghua,Hua, Xiude,Yin, Wei,Shi, Haiyan,Wang, Minghua,Xia, Yazhong,Zhou, Qiujun,Lu, Yiwen,Li, Wei,Liu, Fengquan.

[5]A putative miR172-targeted CeAPETALA2-like gene is involved in floral patterning regulation of the orchid Cymbidium ensifolium. Yang, F. X.,Zhu, G. F.,Wang, Z.,Liu, H. L.,Huang, D.. 2015

[6]Comparative Proteomic Analysis of Labellum and Inner Lateral Petals in Cymbidium ensifolium Flowers. Li, Xiaobai,Xu, Weiwei,Chowdhury, Moytri Roy,Jin, Feng. 2014

[7]Effects of Calcium, 6-Benzyladenine and Paclobutrazol on Inflorescence Quality of Oncidium. Liu, Xiaorong,Liao, Feixiong,Xu, Yechun,Wang, Biqing. 2012

[8]A novel technique for isolating orchid mycorrhizal fungi. Zhu, G. S.,Yu, Z. N.,Gui, Y.,Liu, Z. Y.,Zhu, G. S.,Gui, Y.,Zhu, G. S.,Gui, Y.. 2008

[9]Chemical Variation in Essential Oil of Cymbidium sinense Flowers from Six Cultivars. Li, Jie,Zhu, Gen-fa,Wang, Zai-hua.

[10]An Indirect Enzyme-Linked Immunosorbent Assay for Determination of New Herbicide H-9201 in Water. Zhang Cun-Zheng,Liu Xian-Jin,Yu Jie,Zhang Cun-Zheng,Liu Xian-Jin,Yu Jie,Yang Chun-Long,Chen Min. 2011

[11]Development of an immunochromatographic strip for the detection of antibodies against Porcine circovirus-2. Jin, Qianyue,Wang, Yinbiao,Chen, Wen,Jin, Qianyue,Yang, Jifei,Lu, Qingxia,Guo, Junqing,Deng, Ruiguang,Wang, Yinbiao,Wang, Shihong,Wang, Shufen,Chen, Wen,Zhi, Yubao,Wang, Li,Yang, Suzhen,Zhang, Gaiping,Wang, Shufen,Wang, Shufen. 2012

[12]Validation of a KHV antibody enzyme-linked immunosorbent assay (ELISA). Bergmann, S. M.,Fichtner, D.,Lenk, M.,Reiche, S.,Fuchs, W.,Wang, Q.,Zeng, W.,Li, Y.,Wang, Y.,Zheng, S.,Matras, M.,Reichert, M.,Morin, T.,Olesen, N. J.,Skall, H. F.,Lee, P-Y,Monaghan, S.,Kotler, M.,Way, K.,Braeuer, G.,Boettcher, K.,Kappe, A.,Kielpinska, J..

[13]Quadruple antigenic epitope peptide producing immune protection against classical swine fever virus. Liu, Siguo,Yu, Xinglong,Wang, Chunlai,Wu, Jianmin,Kong, Xiangang,Tu, Changchun.

[14]Synthesis and Identification of Generic Antigen for Ether Pyrethroid Pesticides. Sui Ke,Pan Jia-Rong,Wang Lei,Wang Zhi-Wei,Zhao Jie,Zhen Yue-Ming,Pan Jia-Rong,Wang Zhi-Wei,Zhuge Jie-Jing,Pan Wen-Bin,Wang Zhi-Wei. 2012

[15]A phage-displayed chicken single-chain antibody fused to alkaline phosphatase detects Fusarium pathogens and their presence in cereal grains. Hu, Zu-Quan,Li, He-Ping,Zhang, Jing-Bo,Huang, Tao,Liu, Jin-Long,Xue, Sheng,Liao, Yu-Cai,Li, He-Ping,Huang, Tao,Zhang, Jing-Bo,Liao, Yu-Cai,Wu, Ai-Bo,Liao, Yu-Cai.

[16]Development of an immunochromatographic strip for detection of antibodies against porcine reproductive and respiratory syndrome virus. Li, Huawei,Ji, Pengchao,Zhou, Enmin,Zhang, Gaiping,Yang, Jifei,Bao, Dengke,Hou, Jie,Zhi, Yubao,Yang, Yanyan,Qiao, Songlin,Zhang, Gaiping,Zhang, Gaiping,Li, Huawei.

[17]A combined DNA vaccine provides protective immunity against Mycobacterium bovis and Brucella abortus in cattle. Yu, Da-Hai,Chen, Su-Ting,Cai, Hong,Hu, Xi-Dan,Li, Shu-Xia.

[18]Development and evaluation of an indirect enzyme-linked immunosorbent assay for the detection of antibodies against Campylobacter fetus in cattle. Zhao, Hailing,Liu, Huifang,Du, Yanfen,Liu, Siguo,Ni, Hongbo,Wang, Yong,Wang, Chunlai,Si, Wei,Yang, Jinguo,Ling, Jingkai. 2010

[19]A competitive immunoassay with a surrogate calibrator curve for aflatoxin M-1 in milk. Guan, Di,Li, Peiwu,Zhang, Qi,Zhang, Wen,Guan, Di,Li, Peiwu,Zhang, Wen,Guan, Di,Li, Peiwu,Zhang, Qi,Cui, Yehan. 2011

[20]Development of an immunochromatographic strip for antibody detection of pseudorabies virus in swine. Li, Xiujie,Sun, Yaning,Yang, Suzhen,Wang, Yinbiao,Yang, Jifei,Liu, Yunchao,Jin, Qianyue,Li, Xuewu,Guo, Chengliu,Zhang, Gaiping,Zhang, Gaiping,Li, Xiujie,Yang, Suzhen,Zhang, Gaiping.

作者其他论文 更多>>

微信小程序