The VP37 protein of Broad bean wilt virus 2 induces tubule-like structures in both plant and insect cells

文献类型： 外文期刊

第一作者： Liu, Chengke

作者： Liu, Chengke;Ye, Lufei;Hong, Jian;Zhou, Xueping;Lang, Guojun;Zhang, Chuanxi;Hong, Jian

作者机构：

关键词： Broad bean wilt virus 2;VP37-GFP fusion protein;Tubules;Cell-to-cell movement;BY-2 protoplasts;Insect cells

期刊名称：VIRUS RESEARCH （ 影响因子：3.303； 五年影响因子：3.445 ）

ISSN： 0168-1702

年卷期： 2011 年 155 卷 1 期

页码：

收录情况： SCI

摘要： VP37 protein of Broad bean wilt virus 2 (BBWV-2) is a multifunctional protein that binds single-strand nucleic acids, interacts with viral coat protein (CP) and potentiates the virus cell-to-cell movement in its host plant. In this study, tubule-like structures filled with virus-like particles were observed by Electron Microscopy in plasmodesmata in walls of Chenopodium quinoa leaf cells infected with BBWV-2. Immuno-gold labeling using VP37 protein specific antibody demonstrates that the VP37 is a component of the tubular structures. When VP37 was fused with the green fluorescent protein (VP37-GFP) and expressed in BY-2 protoplasts or in insect Tn cells, green fluorescent tubules of various lengths were produced, protruding from the surface of the expressing cells. These findings suggest that the movement of BBWV-2 between cells is mediated by the tubular structures that contain the VP37 protein, and the VP37 protein itself is capable of inducing these tubule-like structures in cells. Our results also suggest that the plant and insect cell factors involved in the tubule formation have conserved features. (C) 2010 Elsevier B.V. All rights reserved.

分类号： Virology

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