Size-Independent and Noncooperative Recognition of dsRNA by the Rice Stripe Virus RNA Silencing Suppressor NS3

文献类型： 外文期刊

第一作者： Shen, Mei

作者： Shen, Mei;Jia, Ru;Ye, Keqiong;Shen, Mei;Shen, Mei;Xu, Yi;Zhou, Xueping

作者机构：

关键词： cooperativity;dsRNA-binding protein;RNA silencing;viral suppressor;plant-virus interactions

期刊名称：JOURNAL OF MOLECULAR BIOLOGY （ 影响因子：5.469； 五年影响因子：5.826 ）

ISSN： 0022-2836

年卷期： 2010 年 404 卷 4 期

页码：

收录情况： SCI

摘要： Plant and animal viruses employ diverse suppressor proteins to thwart the host antiviral reaction of RNA silencing. Many suppressors bind dsRNA with different size specificity. Here, we examine the dsRNA recognition mechanism of the Rice stripe virus NS3 suppressor using quantitative biochemical approaches, as well as mutagenesis and suppression activity analyses in plants. We show that dimeric NS3 is a size-independent, rather than small interfering RNA-specific, dsRNA-binding protein that recognizes a minimum of 9 bp and can bind to long dsRNA with two or more copies. Global analysis using a combinatorial approach reveals that NS3 dimer has an occluded site size of similar to 13 bp on dsRNA, an intrinsic binding constant of 1 x 10(8) M(-1), and virtually no binding cooperativity. This lack of cooperativity suggests that NS3 is not geared to target long dsRNA. The larger site size of NS3, compared with its interacting size, indicates that the NS3 structure has a border region that has no direct contact with dsRNA but occludes a similar to 4-bp region from binding. We also develop a method to correct the border effect of ligand by extending the lattice length. In addition, we find that NS3 recognizes the helical structure and 2'-hydroxyl group of dsRNA with moderate specificity. Analysis of dsRNA-binding mutants suggests that silencing of the suppression activity of NS3 is mechanistically related to its dsRNA binding ability. (C) 2010 Elsevier Ltd. All rights reserved.

分类号： Biochemistry & Molecular Biology

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