Molecular characterization and experimental host-range of two begomoviruses infecting Clerodendrum cyrtophyllum in China

文献类型: 外文期刊

第一作者: Li, Jing

作者: Li, Jing;Zhou, Xueping

作者机构:

关键词: Clerodendrum cyrtophyllum;Begomovirus;Clerodendrum golden mosaic Jiangsu virus;Clerodendrum golden mosaic China virus;Infectivity

期刊名称:VIRUS GENES ( 影响因子:2.332; 五年影响因子:2.0 )

ISSN: 0920-8569

年卷期: 2010 年 41 卷 2 期

页码:

收录情况: SCI

摘要: Two begomovirus isolates (YX2-I and YX2-II) were identified from Clerodendrum cyrtophyllum showing yellow mosaic symptoms collected in Jiangsu province of China. Sequence analysis reveals that YX2-I is a distinct begomovirus species for which the name Clerodendrum golden mosaic Jiangsu virus (ClGMJSV-[CN:YX2:08]) is proposed. YX2-II is an isolate of bipartite begomovirus Clerodendrum golden mosaic China virus (ClGMCNV-[CN:YX2:08]). Infectious clones of the two viruses were constructed and agroinoculated into Nicotiana benthamiana, N. glutinosa, N. tabacum Samsun, Petunia hybrida, Solanum lycopersicum, Capsicum annuum, S. melongena, Glycine max and Gossypium hirsutum plants. ClGMJSV induced leaf curling and stunting symptoms in N. benthamiana, N. glutinosa, N. tabacum Samsun, and P. hybrida, and ClGMCNV infected N. benthamiana, N. glutinosa, and N. tabacum Samsun with severe symptoms and P. hybrida without obvious symptom. Latent infection in N. benthamiana, N. glutinosa, N. tabacum Samsun, and P. hybrida plants was observed when plants were inoculated with ClGMCNV DNA-A alone. In addition, we illustrated that ClGMCNV DNA-A was capable of interacting with the betasatellite associated with Tobacco curly shoot virus (TbCSB) to produce symptoms in N. benthamiana and N. glutinosa plants, and ClGMJSV could interact with TbCSB but not with ClGMCNV DNA-B in N. benthamiana plants.

分类号: Genetics & Heredity; Virology

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