文献类型： 外文期刊

第一作者： Zhang QinFen

作者： Zhang QinFen;Dai XinHong;Li KunPeng;Xie Li;Hong Jian;Zhou XuePing;Lu XingMeng;Xie Li;Hong Jian

作者机构：

关键词： Infectious flacherie virus;cryo-electron microscopy;three-dimensional structure;polypeptide folding

期刊名称：SCIENCE IN CHINA SERIES C-LIFE SCIENCES （ 影响因子：1.61； 五年影响因子：1.148 ）

ISSN： 1006-9305

年卷期： 2009 年 52 卷 12 期

页码：

收录情况： SCI

摘要： Cryo-electron microscopy and image reconstruction were used to determine the three-dimensional structure of Infectious flacherie virus (IFV). 5047 particles were selected for the final reconstruction. The FSC curve showed that the resolution of this capsid structure was 18 . The structure is a psuedo T=3 (P=3) icosahedral capsid with a diameter of 302.4 and a single shell thickness of 15 . The density map showed that IFV has a smooth surface without any prominent protrude or depression. Comparison of the IFV structure with those of the insect picorna-like virus-Cricket paralysis virus (CrPV)and human picornavirus-Human rhinovirus 14 (HRV 14) revealed that the IFV structure resembles the CrPV structure. The "Rossmann canyon" is absent in both IFV and CrPV particles. The polypeptide topology of IFV VP2, IFV VP3 was predicted and the subunit location at the capsid surface was further analyzed.

分类号： Life Sciences & Biomedicine - Other Topics