Monoclonal antibodies against Tobacco rattle virus and its application in the detection of TRV isolates
文献类型: 外文期刊
第一作者: Wu, Xiaoyun
作者: Wu, Xiaoyun;Zhou, Xueping;Wu, Jianxiang;Wu, Xiaoyun;Li, Zhengyue;Gao, Wenna
作者机构:
关键词: Tobacco rattle virus;monoclonal antibody;TAS-ELISA;IC-RT-PCR
期刊名称:INDIAN JOURNAL OF VIROLOGY ( 影响因子:0.364; 五年影响因子:0.287 )
ISSN: 0970-2822
年卷期: 2009 年 20 卷 2 期
页码:
收录情况: SCI
摘要: Two murine hybridoma cell lines secreting monoclonal antibodies (MAbs) against Tobacco rattle virus (TRV) were produced by fusion of mouse myeloma cells (Sp2/0) with spleen cells from BALB/c mice immunized with purified TRV isolate PpK20. Western blot results indicated that both MAbs could react with the coat proteins of four TRV isolates tested. Triple antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA) was optimized with produced MAbs. Also, the sensitivity of TAS-ELISA was compared with that of indirect-ELISA. Finally, immunocapture RT-PCR (IC-RT-PCR) was established for reliable and efficient detection of-my. The two established assays could be used to detect rapidly and reliably TRV in the field plants.
分类号: Virology
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