Monoclonal antibodies against Tobacco rattle virus and its application in the detection of TRV isolates

文献类型： 外文期刊

第一作者： Wu, Xiaoyun

作者： Wu, Xiaoyun;Zhou, Xueping;Wu, Jianxiang;Wu, Xiaoyun;Li, Zhengyue;Gao, Wenna

作者机构：

关键词： Tobacco rattle virus;monoclonal antibody;TAS-ELISA;IC-RT-PCR

期刊名称：INDIAN JOURNAL OF VIROLOGY （ 影响因子：0.364； 五年影响因子：0.287 ）

ISSN： 0970-2822

年卷期： 2009 年 20 卷 2 期

页码：

收录情况： SCI

摘要： Two murine hybridoma cell lines secreting monoclonal antibodies (MAbs) against Tobacco rattle virus (TRV) were produced by fusion of mouse myeloma cells (Sp2/0) with spleen cells from BALB/c mice immunized with purified TRV isolate PpK20. Western blot results indicated that both MAbs could react with the coat proteins of four TRV isolates tested. Triple antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA) was optimized with produced MAbs. Also, the sensitivity of TAS-ELISA was compared with that of indirect-ELISA. Finally, immunocapture RT-PCR (IC-RT-PCR) was established for reliable and efficient detection of-my. The two established assays could be used to detect rapidly and reliably TRV in the field plants.

分类号： Virology

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