Monoclonal Antibodies against the Recombinant Nucleocapsid Protein of Tomato spotted wilt virus and its Application in Virus Detection
文献类型: 外文期刊
第一作者: Wu, Jianxiang
作者: Wu, Jianxiang;Zhou, Xueping;Yu, Cui;Yang, Cuiyun
作者机构:
关键词: Tomato spotted wilt virus;monoclonal antibodies;virus detection;China
期刊名称:JOURNAL OF PHYTOPATHOLOGY ( 影响因子:1.789; 五年影响因子:1.574 )
ISSN: 0931-1785
年卷期: 2009 年 157 卷 6 期
页码:
收录情况: SCI
摘要: Tomato spotted wilt virus (TSWV) is the type member of the tospovirus genus and causes significant losses in a wide range of economically important ornamental and vegetable crops worldwide. The nucleocapsid gene, located on the ambisense S RNA segment of TSWV was expressed in Escherichia coli using pET-32a as vector and correct expression of recombinant protein was confirmed by Western blot using an anti-TSWV monoclonal antibody (MAb). The recombinant protein was purified using Ni-NTA agarose and the purified protein was used for the production of MAbs. Three murine MAbs against the recombinant nucleocapsid protein were produced. Triple antibody sandwich enzyme-linked immunosorbent assay and immunocapture RT-PCR methods were then established for reliable and efficient detection of TSWV using the produced MAbs.
分类号: Plant Sciences
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