Tomato yellow leaf curl Thailand virus-[Y72] from Yunnan is a monopartite begomovirus associated with DNA beta

文献类型: 外文期刊

第一作者: Guo, Wei

作者: Guo, Wei;Yang, Xiuling;Xie, Yan;Cui, Xiaofeng;Zhou, Xueping

作者机构:

关键词: Tomato yellow leaf curl Thailand virus;Monopartite begomovirus;Evolution

期刊名称:VIRUS GENES ( 影响因子:2.332; 五年影响因子:2.0 )

ISSN: 0920-8569

年卷期: 2009 年 38 卷 2 期

页码:

收录情况: SCI

摘要: Previous studies have shown that isolates of tomato yellow leaf curl Thailand virus (TYLCTHV) originated from Thailand are bipartite begomoviruses, while all the seven TYLCTHV isolates found in China are associated with DNA beta molecules. In this study, infectious clones of TYLCTHV isolate Y72 (TYLCTHV-[Y72]) and its DNA beta were constructed to verify the bipartite or monopartite nature of TYLCTHV. Agroinoculation showed that TYLCTHV-[Y72] alone was able to induce significant symptoms in Nicotiana benthamiana, Nicotiana glutinosa, and Solanum lycopersicum plants, but co-inoculation with its associated satellite DNA beta produced more severe symptoms, which is similar to tobacco curly shoot virus. Southern blot results showed that TYLCTHV DNA beta could increase the virus accumulation in systemically infected tissues. Thus, TYLCTHV-[Y72] is a monopartite begomovirus, which may represent an evolutionary intermediate between the begomoviruses requiring DNA beta and begomoviruses dispensable of DNA beta.

分类号: Genetics & Heredity; Virology

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