Complete nucleotide sequence of a new strain of Tobacco necrosis virus A infecting soybean in China and infectivity of its full-length cDNA clone

文献类型： 外文期刊

第一作者： Xi, Dehui

作者： Xi, Dehui;Li, Jiang;Han, Chenggui;Li, Dawei;Yu, Jialin;Zhou, Xueping

作者机构：

关键词： Tobacco necrosis virus A;soybean strain;complete nucleotide sequence;phylogenetic analysis;infectious cDNA clone

期刊名称：VIRUS GENES （ 影响因子：2.332； 五年影响因子：2.0 ）

ISSN： 0920-8569

年卷期： 2008 年 36 卷 1 期

页码：

收录情况： SCI

摘要： The complete nucleotide sequence of a virus isolated from soybean (Glycine max (L.) Merr.) in China, previously identified as a new strain of Tobacco necrosis virus A (TNV-A) based on its biological, serological properties, and coat protein (CP) sequence and named as TNV-A(C), was determined and compared with that of TNV-A and other closely related Necroviruses and Carmoviruses. The viral RNA genome consists of 3,682 nucleotides and contains five open reading frames (ORFs). TNV-A(C) showed 86.4% overall nucleotide sequence identity to TNV-A. The CP and putative RNA-dependent RNA polymerase (RdRp) showed 88.8 and 95.9% amino acid identity, respectively, to that of TNV-A. The greatest difference between TNV-A(C) and TNV-A was in the 30 terminal region: the p7K ORF region present in TNV-A was absent in TNV-A(C). Phylogenetic analysis of RdRp, CP, and small ORF regions of Necroviruses confirmed TNV-A(C) as a new strain of TNV-A. A full-length cDNA clone of TNV-A(C) was constructed and used as template for run-off transcription based on the obtained sequence. The results indicate that the in vitro-synthesized viral RNA faithfully represented the biological activity of wild-type TNV-A(C).

分类号： Genetics & Heredity; Virology

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