Pathogenicity of a naturally occurring recombinant DNA satellite associated with tomato yellow leaf curl China virus
文献类型： 外文期刊
第一作者： Tao, Xiaorong
作者： Tao, Xiaorong;Zhou, Xueping
作者机构：
期刊名称：JOURNAL OF GENERAL VIROLOGY （ 影响因子：3.891； 五年影响因子：3.719 ）
ISSN： 0022-1317
年卷期： 2008 年 89 卷
页码：
收录情况： SCI
摘要： Recombinant DNA beta molecules (RecDNA-A beta) comprising parts of DNA A and DNA beta associated with tomato yellow leaf curl China virus (TYLCCNV) have been identified in naturally infected tobacco plants. Several examples of the recombinant DNA have been cloned and characterized by sequence analysis. All are approximately half the size of TYLCCNV genomic DNA, and all contain the beta C1 gene and the A-rich region from TYLCCNV DNA beta as well as intergenic region sequences and the 5' terminus of the AC1 gene from TYLCCNV DNA A. RecDNA-A beta was detected by PCR in five of 25 TYLCCNV isolates. Co-inoculation of TYLCCNV DNA A and RecDNA-A beta induced symptoms indistinguishable from those induced by TYLCCNV DNA A and DNA beta in Nicotiana benthamiana, Nicotiana glutinosa, Solarium lycopersicum and Petunia hybrida plants, and Southern blot hybridization results showed that RecDNA-A beta could replicate stably in N. benthamiana plants.
分类号： Biotechnology & Applied Microbiology; Virology
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