Tomato leaf curl Guangxi virus is a distinct monopartite begomovirus species

文献类型： 外文期刊

第一作者： Xu, Youping

作者： Xu, Youping;Cai, Xinzhong;Zhou, Xueping

作者机构：

关键词： tomato;tomato leaf curl Guangxi virus;begomovirus;satellite DNA;DNA ss;recombinant

期刊名称：EUROPEAN JOURNAL OF PLANT PATHOLOGY （ 影响因子：1.907； 五年影响因子：2.022 ）

ISSN： 0929-1873

年卷期： 2007 年 118 卷 3 期

页码：

收录情况： SCI

摘要： Three begomovirus isolates were obtained from tomato plants showing leaf curl symptoms in Guangxi province of China. Typical begomovirus DNA components representing the three isolates (GX-1, GX-2 and GX-3) were cloned and their full-length sequences were determined to be 2752 nucleotides. Nucleotide identities among the three viral sequences were 98.9-99.7%, but all shared < 86.7% nucleotide sequence identity with other reported begomoviruses. The sequence data indicated that GX-1, GX-2 and GX-3 are isolates of a distinct begomovirus species for which the name Tomato leaf curl Guangxi virus (ToLCGXV) is proposed. Further analysis indicated that ToLCGXV probably originated through recombination among viruses related to Ageratum yellow vein virus, Tomato leaf curl China virus and Euphorbia leaf curl virus. PCR and Southern blot analyses demonstrated that isolates GX-1 and GX-2 were associated with DNA beta components, but not isolate GX-3. Sequence comparisons revealed that GX-1 and GX-2 DNA beta components shared the highest sequence identity (86.2%) with that of Tomato yellow leaf curl China virus (TYLCCNV). An infectious construct of ToLCGXV isolate GX-1 (ToLCGXV-GX) was produced and determined to be highly infectious in Nicotiana benthamiana, N. glutinosa, tobacco cvs. Samsun and Xanthi, tomato and Petunia hybrida plants inducing leaf curl and stunting symptoms. Co-inoculation of tomato plants with ToLCGXV-GX and TYLCCNV DNA beta resulted in disease symptoms similar to that caused by ToLCGXV-GX alone or that observed in infected field tomato plants.

分类号： Agriculture; Plant Sciences

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