Efficient gene silencing induction in tomato by a viral satellite DNA vector

文献类型： 外文期刊

第一作者： Cai, Xinzhong

作者： Cai, Xinzhong;Wang, Changchun;Xu, Youping;Xu, Qiufang;Zheng, Zhong;Zhou, Xueping

作者机构：

关键词： Tomato yellow leaf curl China virus;DNA beta;geminivirus;virus-induced gene silencing;temperature;tomato

期刊名称：VIRUS RESEARCH （ 影响因子：3.303； 五年影响因子：3.445 ）

ISSN： 0168-1702

年卷期： 2007 年 125 卷 2 期

页码：

收录情况： SCI

摘要： Virus-induced gene silencing (VIGS) in tomato (Lycopersicon esculentum Mill.) is currently routinely analysed using Tobacco rattle virus (TRV)based vector. We recently reported a new vector system modified from DNA beta (DNAm beta) of Tomato yellow leaf curl China virus (TYLCCNV) for VIGS analysis in Solanaceous species including tomato. Here, we describe DNAm beta-induced gene silencing in tomato. We found that DNAm beta-induced gene silencing was initiated from vascular tissues, and later scattered to other tissues. Once initiated in seedlings, the silencing phenotype lasted for the entire life span of the plants, was expressed in a variety of tissues and organs including leaf, shoot, stem, flower and fruit, and could be achieved at any growth stage. It was insensitive to temperature as high as 32 degrees C and no symptoms were observed in silenced plants. The DNAm beta vector worked efficiently in at least seven tomato cultivars, indicating that this system has great potential as a versatile VIGS system for routine functional analysis of genes in tomato. (c) 2007 Elsevier B.V. All rights reserved.

分类号： Virology

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