Modification of a viral satellite DNA-based gene silencing vector and its application to leaf or flower color change in Petunia hybrida

文献类型: 外文期刊

第一作者: Tao Xiaorong

作者: Tao Xiaorong;Qian Yajuan;Zhou Xueping

作者机构:

关键词: geminivirus;satellite DNA;DNA beta;virus induced gene silencing;petunia;Su;CHS

期刊名称:CHINESE SCIENCE BULLETIN ( 影响因子:1.649; 五年影响因子:1.738 )

ISSN: 1001-6538

年卷期: 2006 年 51 卷 18 期

页码:

收录情况: SCI

摘要: Virus-induced gene silencing offers a powerful reverse-genetic tool for the study of gene function in plants. We have previously reported effective gene silencing of plant genes using a viral satellite DNA associated with Tomato yellow leaf curl China virus (TYLCCNV). In this study, we further modified the viral satellite DNA-based vector. The modified vector can induce sulfu (Su) gene silencing as effective as the original vector in Nicotiana benthamiana plants, but the new system simplifies procedures for construction of vector derivative. Furthermore, a fragment of petunia Su or chalcone synthase (CHS) endogenous gene was inserted into the modified vector. When petunia plants were agroinoculated with the modified vector carrying a Su or CHS gene, the Su silenced plants started to appear yellowing in veins of systemically infected upper leaves two weeks after agroinoculation, while the CHS silenced plants started to show flower color change one month after agroinoculation and later single-color flowers became mosaic.

分类号: Science & Technology - Other Topics

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