Whole-Genome Resequencing-Based Qualitative Trait Locus Mapping Correlated yellow with the Mutant Color in Honeybees, Apis cerana cerana

文献类型： 外文期刊

第一作者： Shao, Shanshan

作者： Shao, Shanshan;Huang, Qiang;Pei, Yalin;Hu, Junyan;Wang, Zilong;Zhang, Lizhen;He, Xujiang;Wu, Xiaobo;Yan, Weiyu;Shao, Shanshan;Huang, Qiang;Pei, Yalin;Hu, Junyan;Wang, Zilong;Zhang, Lizhen;He, Xujiang;Wu, Xiaobo;Yan, Weiyu;Shao, Shanshan

作者机构：

关键词： Apis cerana cerana; resequencing; brown mutation; yellow; RNA interference

期刊名称：ANIMALS （ 影响因子：3.0； 五年影响因子：3.2 ）

ISSN： 2076-2615

年卷期： 2024 年 14 卷 6 期

页码：

收录情况： SCI

摘要： The honeybee, Apis cerana cerana (Ac), is an important pollinator and has adapted to the local ecological environment with relevant coloration. The cuticle coloration of the brown (br) mutant is brown instead of black in wild-type individuals. Therefore, this study aimed to identify and characterize the gene responsible for the br mutation. Genome resequencing with allele segregation measurement using Euclidean distance followed by Lowess regression analysis revealed that the color locus linked to the mutation was located on chromosome 11. A 2-base deletion on exon 4 was identified in the g7628 (yellow) gene after genome assembly and sequence cloning. In addition, the cuticle color of the abdomen of worker bees changed from black to brown when a defect was induced in the yellow gene using short interfering RNA (siRNA); however, the survival rate did not decrease significantly. These results indicate that the yellow gene participated in the body pigmentation, and its defect was responsible for the br mutation. This study promotes the understanding of the molecular basis of body coloration in honeybees, enriching the molecular mechanisms underlying insect pigmentation.

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