文献类型： 外文期刊

第一作者： Zhang Yan-li

作者： Zhang Yan-li;Dong Hui;Gu Zu-min;Zhou Jin-cheng;Zhang Li-sheng;Zhou Jin-cheng

作者机构：

关键词： biopesticide; fall armyworm; invasive pest; biological control agent; Metarhizium rileyi

期刊名称：JOURNAL OF APPLIED ENTOMOLOGY （ 影响因子：2.183； 五年影响因子：2.442 ）

ISSN： 0931-2048

年卷期： 2022 年 146 卷 6 期

页码：

收录情况： SCI

摘要： The fall armyworm (FAW), Spodoptera frugiperda, is a new and dangerous invasive pest in many countries. Entomopathogenic fungi (EPF) have been considered as potential biological control agents against the FAW. In this study, eight natural fungal strains were isolated from diseased larvae of FAW, and identified as Cladosporium cladosporioides (EFNL-1), Metarhizium rileyi (EFNL-2 and EFNL-8), Fusarium proliferatum (EFNL-3 and EFNL-6), F. equiseti (EFNL-4 and EFNL-5) and F. tricinctum (EFNL-7), using the morphological and molecular tools or methods. The instantaneous death risk and mortality of 3(rd)-instar larvae inoculated with EFNL-8 (1 x 10(8) conidia/ml) were significantly higher than for any other fungal isolates and for the control group. Thereafter, we tested the pathogenicity of EFNL-8 (1 x 10(7) conidia/ml) against 1(st)- to 4(th)-larvae, pupae, and eggs of FAW. The instantaneous death risk and mortality of 1(st)-instar (Median lethal time = 9.02 days; Corrected motality = 88.68%), 2(nd)-instar (12.00 days; 62.50%) and 3(rd)-instar larvae (13.79 days; 56.67%) inoculated with EFNL-8 were significantly higher than that of larvae in the control groups. When the eggs of FAW were inoculated with EFNL-8 (1 x 10(7) conidia/ml), EFNL-8 caused eggs (56.62%), and newly hatched neonates after 3 days (68.75%) and 6 days (80.60%) mortality, compared with the control groups. However, the mortality of the EFNL-8 isolate to 4(th)-instar larvae (5.00%) and pupae (14.81%) was low and did not differ from the control groups. This study identified M. rileyi EFNL-8 as a pathogenic isolate against eggs and 1(st)- to 3(rd)-instar larvae of FAW. However, further studies including field validation of this EPF biopesticide candidate should be undertaken in newly invaded areas by the pest.

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