Identification and Genomic Analyses of a Multidrug Resistant Avian Pathogenic Escherichia coli Coharboring mcr-1, blaTEM-176 and blaCTX-M-14 Genes

文献类型： 外文期刊

第一作者： Wang, Zhiyang

作者： Wang, Zhiyang;Wang, Xinyu;Guo, Weiqi;Wang, Di;Hu, Jiangang;Zhang, Beibei;Qi, Jingjing;Tian, Mingxing;Bao, Yanqing;Wang, Shaohui;Wang, Zhiyang;Li, Haihua;Wang, Shaohui

作者机构：

期刊名称：TRANSBOUNDARY AND EMERGING DISEASES （ 影响因子：4.3； 五年影响因子：4.3 ）

ISSN： 1865-1674

年卷期： 2024 年 2024 卷

页码：

收录情况： SCI

摘要： The emergence and transmission of the colistin-resistance gene mcr and extended-spectrum beta-lactamase (ESBL) encoding genes pose a significant threat to global public health. In recent years, it has been reported that mcr-1 and ESBL genes can coexist in single bacteria strain. The objective of this study was to characterize a multidrug-resistant (MDR) avian pathogenic Escherichia coli (APEC) isolate carrying mcr and ESBL encoding genes in China. A total of 200 APEC isolates were collected for antimicrobial susceptibility testing by Kirby-Bauer (K-B) disk method. The MDR strain EC012 were then further analyzed for minimum inhibitory concentrations, antimicrobials resistance genes (ARGs) detection, conjugation, and whole-genome sequencing (WGS). Among all APEC isolates determined by K-B disk method, strain EC012 was resistant to almost all the antimicrobials, including polymyxin B, cefotaxime, and ceftazidime. Moreover, EC012 harbored ARGs mcr-1, bla(TEM-176), and bla(CTX-M-14). WGS analysis revealed that EC012 belonged to epidemic APEC serotype O1:H16 and multilocus sequence type ST295. EC012 consisted of one chromosome and six plasmids, encoding a broad ARGs. The bla(CTX-M-14), mcr-1 or bla(TEM-176) genes were located on conjugative plasmids pEC012-1 or pEC012-5, respectively. These plasmids were successfully transferred to transconjugants and resulted in the resistance to polymyxin B, cefotaxime, and ceftazidime. This study indicated that APEC was a potential reservoir of colistin-resistance gene mcr-1 and ESBL encoding genes, and highlighted the necessity for enhanced monitoring of ARGs dissemination among bacteria from different origins.

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