Genome-Based Analysis of Genetic Diversity, Antimicrobial Susceptibility, and Virulence Gene Distribution in Salmonella Pullorum Isolates from Poultry in China

文献类型： 外文期刊

第一作者： Cheng, Yiluo

作者： Cheng, Yiluo;Huang, Qi;Zhou, Rui;Cheng, Yiluo;Zhang, Jigao;Luo, Qingping;Zhang, Tengfei

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关键词： Salmonella enterica serovar Pullorum; antibiotic resistance; antibiotic-resistance gene; virulence gene; antigenic type

期刊名称：ANIMALS （ 影响因子：2.7； 五年影响因子：3.2 ）

ISSN： 2076-2615

年卷期： 2024 年 14 卷 18 期

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收录情况： SCI

摘要： Simple Summary: Salmonella enterica serovar Pullorum (S. Pullorum) is a formidable pathogen within the poultry industry and constitutes a substantial threat to global poultry production. Pullorum disease eradication and antibiotic treatment are two main strategies for controlling this disease. Therefore, understanding the prevalence and resistance characteristics of S. Pullorum is important for its control. The objective of this study was to determine the antigenic types and genotype characteristics of S. Pullorum isolates from China, and to clarify the relationship between antibiotic resistance and resistance genes. In this study, all the isolates tested were of standard antigenic types, with ST92 the predominant genotype, and 92.5% of the isolates displayed multidrug resistance. Our analysis of virulence genes indicated that the isolates expressed numerous factors associated with secretion systems. These data provide valuable information for the prevention and control of S. Pullorum infections in the poultry industry. Pullorum disease, caused by Salmonella enterica serovar Pullorum (S. Pullorum) infection, is a major pathogenic threat to the poultry industry. In this study, 40 S. Pullorum isolates from seven provinces of China were comprehensively analyzed in terms of antigenic type and antimicrobial susceptibility, and their drug-resistance genes and virulence genes were identified with whole-genome sequencing (WGS). We show that all these isolates were standard antigenic types, with ST92 the predominant genotype (92.5%). Disk diffusion assays revealed high resistance rates to streptomycin (92.5%), ciprofloxacin (82.5%), and ampicillin (80%), and the resistance rates to streptomycin, gentamicin, ampicillin, and cefotaxime were higher in isolates from sick chickens than in those from healthy chickens. In addition, gyrA mutations and eight acquired resistance genes were identified, with aac(6 ')-Iaa the most prevalent, followed by blaTEM1 beta, sul2, and the GyrA S83F mutation. The resistance phenotypes to streptomycin, ampicillin, and ciprofloxacin correlated strongly with the presence of the aac(6 ')-Iaa resistance gene, blaTEM1 beta resistance gene, and gyrA mutations, respectively. Analysis of the virulence genes showed that the isolates expressed numerous factors associated with secretion systems, including SPI-1 and SPI-2. Overall, this study extends our understanding of the epidemiology and antibiotic resistance of S. Pullorum in China.

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