Stability is essential for insecticidal activity of Vip3Aa toxin against Spodoptera exigua

文献类型： 外文期刊

第一作者： Xu, Lian

作者： Xu, Lian;Zheng, Mei-Xia;Zhu, Yu-Jing;Fu, Bai-Wen;Chen, Qing-Xi;Shi, Yan

作者机构：

关键词： Vip3A; Proteolysis; Molecular dynamics simulation; Insecticidal activity

期刊名称：AMB EXPRESS （ 影响因子：4.126； 五年影响因子：4.245 ）

ISSN： 2191-0855

年卷期： 2022 年 12 卷 1 期

页码：

收录情况： SCI

摘要： Vegetative insecticidal proteins 3A (Vip3A) were important insecticidal proteins for control of lepidopteran pests. Previous study demonstrated that Vip3Aa and Vip3Ad showed significant difference in insecticidal activities against Spodoptera exigua, while the molecular mechanism remained ambiguous. Here we demonstrated that the difference in insecticidal activities between Vip3Aa and Vip3Ad might be caused by the difference in stability of Vip3Aa and Vip3Ad in S. exigua midgut protease. Vip3Aa was quite stable while Vip3Ad could be further degraded. Molecular dynamics simulation revealed that Vip3Aa was more stable than Vip3Ad, with smaller RMSD and RMSF value. Amino acid sequence alignment indicated that three were three extra prolines (P591, P605 and P779) located on Vip3Aa. We further identified that residue P591 played a crucial role on stability and insecticidal activity of Vip3Aa. Taken together, our study demonstrated that the stability was essential for the insecticidal activity of Vip3A toxins, which might provide new insight into the action mode of Vip3A toxins and contribute to the design Vip3A variants with improved stability and insecticidal activity.

分类号：

  • 相关文献
作者其他论文 更多>>
  • Comparative phylogenomics and phylotranscriptomics provide insights into the genetic complexity of nitrogen-fixing root-nodule symbiosis

    作者：Zhang, Yu;Fu, Yuan;Xian, Wenfei;Li, Xiuli;Feng, Yong;Bu, Fengjiao;Shi, Yan;Chen, Shiyu;Cheng, Shifeng;van Velzen, Robin;Schranz, M. Eric;Battenberg, Kai;Berry, Alison M.;Salgado, Marco G.;Pawlowski, Katharina;Liu, Hui;Yi, Tingshuang;Fournier, Pascale;Alloisio, Nicole;Pujic, Petar;Schranz, M. Eric;Normand, Philippe;Delaux, Pierre-Marc;Wong, Gane Ka-Shu;Hocher, Valerie;Svistoonoff, Sergio;Gherbi, Hassen;Wang, Ertao;Kohlen, Wouter;Wall, Luis G.;Parniske, Martin;Doyle, Jeffrey J.;Fu, Yuan;Fu, Yuan

    关键词：nitrogen-fixing root-nodule symbiosis; two competing hypotheses; phylogenomics; phylotranscriptomics; conserved non-coding elements; convergence; deep homology

  • PsNRT2.3 interacts with PsNAR to promote high-affinity nitrate uptake in pea (Pisum sativum L.)

    作者：Chen, Baizhi;Shi, Yan;Lu, Lu;Wang, Luyao;Sun, Yuchen;Ning, Weidong;Liu, Zijian;Cheng, Shifeng;Lu, Lu;Lu, Lu;Wang, Luyao

    关键词：Pea; Nitrate; Nitrate transporter 2; Nitrogen assimilation related proteins

  • Oral phages prophylaxis against mixed Escherichia coli O157:H7 and Salmonella Typhimurium infections in weaned piglets

    作者：Li, Yinan;Li, Xun;Hu, Chuanhuo;Wang, Xiaoye;Zhang, Lili;Li, Yinan;Li, Xun;Hu, Chuanhuo;Wang, Xiaoye;Li, Lei;Han, Kaiou;Mao, Xinyu;Wang, Leping;Cao, Yajie;Wu, Yuxing;Tan, Yizhou;Shi, Yan;Liu, Hui;Peng, Hao;Li, Lei;Han, Kaiou;Mao, Xinyu;Wang, Leping;Cao, Yajie;Wu, Yuxing;Tan, Yizhou;Shi, Yan;Liu, Hui;Li, Yinan;Peng, Hao;Li, Xun;Hu, Chuanhuo;Wang, Xiaoye;Li, Yinan;Peng, Hao;Li, Xun;Hu, Chuanhuo;Wang, Xiaoye;Wang, Xiaoye

    关键词：Oral; Microencapsulated phage; Prophylaxis; Escherichia coli; Salmonella Typhimurium

  • Engineering of Bacillus thuringiensis Cry2Ab toxin for improved insecticidal activity

    作者：Xu, Lian;Zheng, Mei-Xia;Zhu, Yu-Jing;Fu, Bai-Wen;Shi, Yan

    关键词：Cry2Ab; Protein engineering; Oligomerization; Pore-forming activity; Insecticidal activity

  • Barley yellow dwarf virus-GAV 17K protein disrupts thiamine biosynthesis to facilitate viral infection in plants

    作者：Han, Xiaoyu;Yang, Xue;Chen, Siyu;Wang, He;Chen, Linlin;Sun, Bingjian;Li, Honglian;Shi, Yan;Liu, Xiaomin;Wang, Daowen;Yang, Jin;Li, Honglian;Shi, Yan

    关键词：barley yellow dwarf virus; 17K; thiamine; pyrimidine synthase (THIC); transcript profiling

  • Harnessing landrace diversity empowers wheat breeding

    作者：Cheng, Shifeng;Feng, Cong;Cheng, Hong;Jiang, Mei;Huang, Zejian;Chen, Baizhi;Han, Ruirui;Hou, Junliang;Iqbal, Muhammad S.;Liu, Hongbing;Liu, Shiyan;Ma, Lingling;Sarfraz, Zareen;Shi, Yan;Song, Bo;Waheed, Abdul;Wang, Xingwei;Wei, Yanping;Wu, Shishi;Wu, Yubing;Xian, Wenfei;Xu, Yawen;Xu, Yunfeng;Yuan, Quan;Zhang, Xin;Huang, Sanwen;Wingen, Luzie U.;Leverington-Waite, Michelle;Collier, Sarah;Orford, Simon;Wang, Xiaoming;Awal, Rajani;O'Hara, Tom;Lister, Clare;Siluveru, Ajay;Quiroz-Chavez, Jesus;Ramirez-Gonzalez, Ricardo H.;Bilham, Lorelei;Brown, James K. M.;Chartrain, Laetitia;Gaurav, Kumar;Hafeez, Amber N.;Horler, Richard;Kowalski, Ania;Mumford, Cathy;Philp, Charlie;Playford, Darryl;Steuernagel, Burkhard;Wulff, Brande B. H.;Nicholson, Paul;Chayut, Noam;Uauy, Cristobal;Sanders, Dale;Griffiths, Simon;Riche, Andrew B.;Castle, March;Lovegrove, Alison;Parmar, Saroj;Shewry, Peter R.;Steele, David;Hawkesford, Malcolm J.;Wang, Xiaoming;Barker, Gary;Burridge, Amanda;Przewieslik-Allen, Alexandra M.;Winfield, Mark;Edwards, Keith J.;Bryant, Ruth;Burt, Chris;Schafer, David;Berry, Simon;Tailby, Phillip;Bansal, Urmil;Bariana, Harbans S.;Bariana, Harbans S.;Bennett, Malcolm J.;Foulkes, John;Gaju, Oorbessy;Bicego, Breno;Slafer, Gustavo A.;Buurman, Milika;Denbel, Worku;Elkot, Ahmed F.;Fenwick, Paul;Feuerhelm, David;Gauley, Adam;Dixon, Laura;Gauley, Adam;Han, Ruirui;Kerton, Matthew;Kondic-Spica, Ankica;Lage, Jacob;Li, Xiaolong;Slafer, Gustavo A.;Song, Baoxing;Tyrrell, Simon;Wamalwa, Mercy N.;Wu, Yubing;Xu, Yawen;Zhang, Xin;Wulff, Brande B. H.;Xian, Wenfei;Huang, Sanwen

    关键词：

  • Fluoro-functionalized plant biomass adsorbent: Preparation and application in extraction of trace perfluorinated compounds from environmental water samples

    作者：Tan, Xi;Shi, Yan;Ma, Chun-Feng;Chi, Quan;Yang, Yu-Hang;Xiao, Hua-Ming;Wang, Xian;Zhang, Wen-Xiang;Xiao, Hua-Ming

    关键词：Fluoro-functionalized biomass; Perfluorinated compounds (PFCs); Adsorption efficiency; Mass spectrometry (MS); Fluorinated cedar slag (FCS)

微信小程序