文献类型： 外文期刊
第一作者： Wu, Lina
作者： Wu, Lina;Xu, Shiqi;Huang, Xingxu;Huang, He;Wu, Lina;Huang, He;Wang, Xinjie;Liu, Ming;Cao, Xizhong;Tang, Taishan
作者机构：
关键词： MnO2 nanorods; CRISPR-Cas12a; SARS-CoV-2; Nanozyme; Detection
期刊名称：ACS APPLIED MATERIALS & INTERFACES （ 影响因子：10.383； 五年影响因子：10.382 ）
ISSN： 1944-8244
年卷期：
页码：
收录情况： SCI
摘要： The CRISPR-Cas system was developed into a molecular diagnostic tool with high sensitivity, low cost, and high specificity in recent years. Colorimetric assays based on nanozymes offer an attractive point-of-care testing method for their low cost of use and user-friendly operation. Here, a MnO2 nanozyme-mediated CRISPR-Cas12a system was instituted to detect SARS-CoV2. MnO2 nanorods linked to magnetic beads via a single-stranded DNA (ssDNA) linker used as an oxidase-like nanozyme inducing the color change of 3,3',5,5'-tetramethylbenzidine, which can be distinguished by the naked eye. The detection buffer color will change when the Cas12a is activated by SARS-CoV-2 and indiscriminately cleave the linker ssDNA. The detection limit was 10 copies per microliter and showed no cross-reaction with other coronaviruses. The nanozyme-mediated CRISPR-Cas12a system shows high selectivity and facile operation, with great potential for molecular diagnosis in point-of-care testing applications.
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