Unlocking the antibacterial activity and mechanisms of ε-poly-l-Lysine and perilla combination against Pseudomonas fluorescens: Insights from non-targeted metabolomic analyses

文献类型： 外文期刊

第一作者： Lin, Yile

作者： Lin, Yile;Wang, Wensi;Li, Jie;Ai, Youwei;Wang, Hongxun;Han, Yahong;Hou, Wenfu;Lin, Yile;Wang, Wensi;Wang, Wen;Wang, Hongxun;Hou, Wenfu

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关键词： Pseudomonas fluorescens; epsilon-PL; Perilla; Antibacterial mechanism; Metabolome

期刊名称：LWT-FOOD SCIENCE AND TECHNOLOGY （ 影响因子：6.6； 五年影响因子：6.9 ）

ISSN： 0023-6438

年卷期： 2024 年 208 卷

页码：

收录情况： SCI

摘要： Perilla frutescens extract has been acknowledged for its robust antibacterial properties, while epsilon-Poly-Lysine (epsilon-PL) stands as a naturally occurring food preservative. However, their combined antibacterial activity and mechanisms has not been fully understood. Therefore, the aim of this study was to elucidate the combined antibacterial activity of the epsilon-PL/Perilla combination against Pseudomonas fluorescens. The findings unveiled a significant antibacterial efficacy of epsilon-PL and perilla against P. fluorescens, respectively. Upon treatment with epsilon-PL and perilla, a decline in membrane potential (MP) was observed. These alterations were further accompanied by reductions in nucleic acid content, metabolic activity, and respiratory chain dehydrogenase activity. And by morphological observation, it was found that the cells treated with perilla or epsilon-PL were severely broken. The metabolomic analysis following treatment with epsilon-PL and perilla unveiled substantial shifts in a multitude of metabolites. Multivariate statistical analysis showed that epsilon-PL and perilla hindered nucleotide metabolism and interfered with carbohydrate, amino acid, and lipid metabolism, impacting cell wall biosynthesis, cell membrane structure, and genetic material expression, leading to growth inhibition and bacterial demise. Collectively, these outcomes provide fresh insights into the intricate mechanisms underlying the inhibitory action of epsilon-PL and perilla against P. fluorescens.

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