miR-7002-5p targets caspase-1 to suppress the inflammatory response of macrophages induced by Streptococcus equi subsp. zooepidemicus

文献类型： 外文期刊

第一作者： Xie, Honglin

作者： Xie, Honglin;Deng, Jingfei;Yu, Jingyu;Li, Shun;Wang, Yibo;Li, Yajuan;Huang, Yunfei;Sun, Qinqin;Liao, Jiedan;Deng, Ziteng;Fu, Qiang;Xie, Honglin;Li, Yajuan;Huang, Yunfei;Sun, Qinqin;Liao, Jiedan;Deng, Ziteng;Fu, Qiang;Li, Shun

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关键词： Streptococcus equi subsp. zooepidemicus; Macrophages; miR-7002-5p; Caspase-1; Inflammatory

期刊名称：ARCHIVES OF MICROBIOLOGY （ 影响因子：2.6； 五年影响因子：2.8 ）

ISSN： 0302-8933

年卷期： 2025 年 207 卷 9 期

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收录情况： SCI

摘要： Streptococcus equi subsp. zooepidemicus (SEZ) is an important zoonotic pathogen that causes severe inflammatory diseases in various animal species. The host inflammatory response is a key factor in SEZ pathogenesis, yet the regulatory role of microRNAs (miRNAs) in this process remains largely unexplored. In this study, we investigated the function of miR-7002-5p in SEZ-induced inflammation using murine macrophage J774A.1 cells and C57BL/6J mice. SEZ infection led to a significant downregulation of miR-7002-5p and upregulation of inflammatory cytokines. Bioinformatics prediction and dual-luciferase reporter assays confirmed that Caspase-1 is a direct target of miR-7002-5p. Overexpression of miR-7002-5p significantly suppressed Caspase-1 activation and reduced the expression of IL-1 beta, IL-18, IL-6, and TNF-alpha both in vitro and in vivo. In contrast, inhibition of miR-7002-5p exacerbated inflammatory responses. Furthermore, intranasal delivery of miR-7002-5p mimics in SEZ-infected mice alleviated lung inflammation, as evidenced by reduced cytokine levels and histopathological improvement. These findings suggest that miR-7002-5p mitigates SEZ-induced inflammation by targeting Caspase-1 and may serve as a potential therapeutic target for controlling SEZ infection.

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