Vitamin D3 promotes fish oocyte development by directly regulating gonadal steroid hormone synthesis

文献类型： 外文期刊

第一作者： Yang, Gang

作者： Yang, Gang;Cheng, Ke;Wang, Chunfang;Huang, Yanqing

作者机构：

关键词： vitamin D-3; vitellogenin synthesis; ovarian development; reproduction; zebrafish

期刊名称：BIOLOGY OF REPRODUCTION （ 影响因子：3.6； 五年影响因子：4.2 ）

ISSN： 0006-3363

年卷期： 2024 年 110 卷 3 期

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收录情况： SCI

摘要： Vitamin D receptors and vitamin D-3-metabolizing enzymes have been found to be highly expressed in the ovaries and spermatophores of fish. However, the role of vitamin D-3 on fish gonadal development has rarely been reported. In this study, 2-month-old female zebrafish were fed with different concentrations of vitamin D-3 diets (0, 700, 1400, and 11 200 IU/kg) to investigate the effects of vitamin D-3 on ovarian development. The diet with 0 IU/kg vitamin D-3 resulted in elevated interstitial spaces, follicular atresia, and reproductive toxicity in zebrafish ovaries. Supplementation with 700 and 1400 IU/kg of vitamin D-3 significantly increased the oocyte maturation rate; upregulated ovarian gonadal steroid hormone synthesis capacity; and elevated plasma estradiol, testosterone, and ovarian vitellogenin levels. Furthermore, the current study identified a vitamin D response element in the cyp19a1a promoter and demonstrated that 1.25(OH)(2)D-3-vitamin D response directly activated cyp19a1a production through activating the vitamin D response element. In conclusion, this study shows that an appropriate concentration of vitamin D-3 can promote zebrafish ovarian development and affect vitellogenin synthesis through the vdr/cyp19a1a/er/vtg gene axis.

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