Molecular characterization of a novel photoperiod-insensitive allele Ppd-B1a.3 and its effect on heading date in Chinese wheat (Triticum aestivum) cultivar Qingchun 37

文献类型： 外文期刊

第一作者： Song, Tianqi

作者： Song, Tianqi;Shi, Caiyin;Wang, Yukun;Guo, Sihai;Zhou, Jianfei;Bu, Yaning;Li, Siyi;Fan, Qiru;Wei, Fan;Zhang, Xiaoke;Zhang, Weijun;Chen, Dongsheng;Wang, Xiaoxing;Xiang, Jishan;Wang, Xiaoxing;Xiang, Jishan

作者机构：

关键词： Allelic variant; Heading date; Photoperiod gene; Ppd-B1a.3; Wheat (Triticum aestivum)

期刊名称：JOURNAL OF PLANT RESEARCH （ 影响因子：2.3； 五年影响因子：2.9 ）

ISSN： 0918-9440

年卷期： 2024 年

页码：

收录情况： SCI

摘要： Breeders adjust wheat heading dates to improve regional adaptability and reduce or mitigate yield losses caused by meteorological disasters, pests and diseases. The Ppd-1 genes play a crucial role in determining wheat sensitivity to changes in day-length and serve as key regulators of heading dates once the vernalization requirement is satisfied. In this study, we identified a new allelic variant of the promoter region, Ppd-B1a.3, in the Chinese wheat cultivar Qingchun 37. Compared to the Ppd-B1b.1 (carried by Chihokukomugi), the main mutation sites in Ppd-B1a.3 include a substitution of C with G at the -505-bp, a T base insertion at the -625-bp, a mutation of TCG to GGT at the -632 to -634-bp, and a 163-bp insertion at the -691 bp. Analysis of F2 populations indicated that Ppd-B1a.3 promotes heading and flowering (approximately 6 days earlier in population 1 and 17 days in population 2) under short-day conditions in a greenhouse. However, the evaluation of Ppd-B1a.3's effect under field conditions may be influenced by the copy number of the Ppd-B1 locus inherited from the other parent in the F2 populations. Ppd-B1a.3 disrupts circadian rhythm expression and exhibits a stronger effect on heading and flowering than the three-copy Ppd-B1 allele carried by Jing 411. Origin analysis suggests that Ppd-B1a.3 may have derived from non-native germplasm. These results deepen our understanding of wheat photoperiod genes and provide useful genetic resources for fine-tuning wheat heading dates during breeding.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序