Increased glycine betaine synthesis and salinity tolerance in AhCMO transgenic cotton lines

文献类型： 外文期刊

第一作者： Dong, Hezhong

作者： Dong, Hezhong;Li, Weijiang;Kong, Xiangqiang;Zhang, Huijun;Sun, Yi;Chen, Shouyi

作者机构：

关键词： salinity tolerance

期刊名称：MOLECULAR BREEDING （ 影响因子：2.589； 五年影响因子：2.75 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Glycine betaine is an osmoprotectant that plays an important role and accumulates rapidly in many plants during salinity or drought stress. Choline monooxygenase (CMO) is a major catalyst in the synthesis of glycine betaine. In our previous study, a CMO gene (AhCMO) cloned from Atriplex hortensis was introduced into cotton (Gossypium hirsutum L.) via Agrobacterium mediation to enhance resistance to salinity stress. However, there is little or no knowledge of the salinity tolerance of the transgenic plants, particularly under saline-field conditions. In the present study, two transgenic AhCMO cotton lines of the T-3 generation were used to study the AhCMO gene expression, and to determine their salinity tolerance in both greenhouse and field under salinity stress. Molecular analysis confirmed that the transgenic plants expressed the AhCMO gene. Greenhouse study showed that on average, seedlings of the transgenic lines accumulated 26 and 131% more glycine betaine than those of non-transgenic plants (SM3) under normal and salt-stress (150 mmol l(-1) NaCl) conditions, respectively. The osmotic potential, electrolyte leakage and malondialdehyde (MDA) accumulation were significantly lower in leaves of the transgenic lines than in those of SM3 after salt stress. The net photosynthesis rate and Fv/Fm in transgenic cotton leaves were less affected by salinity than in non-transgenic cotton leaves. Therefore, transgenic cotton over-expressing AhCMO was more tolerant to salt stress due to elevated accumulation of glycine betaine, which provided greater protection of the cell membrane and photosynthetic capacity than in non-transgenic cotton. The seed cotton yield of the transgenic plants was lower under normal conditions, but was significantly higher than that of non-transgenic plants under salt-stressed field conditions. The results indicate that over-expression of AhCMO in cotton enhanced salt stress tolerance, which is of great value in cotton production in the saline fields.

分类号： Q94

  • 相关文献

[1]Genetic improvement of cotton tolerance to salinity stress. Ma, Xinrong,Dong, Hezhong,Li, Weijiang,Ma, Xinrong. 2011

[2]High-Density Linkage Map Construction and Mapping of Salt-Tolerant QTLs at Seedling Stage in Upland Cotton Using Genotyping by Sequencing (GBS). Latyr Diouf,Du, Xiongming,Zhaoe Pan,Shou-Pu He,Wen-Fang Gong,Yin Hua Jia,Richard Odongo Magwanga,Kimbembe Romesh Eric Romy,Harun or Rashid,Joy Nyangasi Kirungu,Xiongming Du. 2017

[3]Differential expression of salt tolerance related genes in Brassica campestris L. ssp chinensis (L.) Makino var. communis Tsen et Lee. Qiu, Yang,Li, Xi-xiang,Zhi, Hai-ying,Shen, Di,Lu, Peng. 2009

[4]Effect of Salt Stress on Growth and Physiology in Melia azedarach Seedlings of Six Provenances. Xu, Liping,Xu, Liping,Zhang, Zihan,Yu, Fangyuan,Guo, Jie,Liu, Jianbin,Yue, Haiwang. 2018

[5]Natural variation of salinity response, population structure and candidate genes associated with salinity tolerance in perennial ryegrass accessions. Tang, Jinchi,Yu, Xiaoqing,Camberato, James J.,Jiang, Yiwei,Luo, Na,Xiao, Fangming. 2013

[6]Enhancement of Salinity Tolerance during Rice Seed Germination by Presoaking with Hemoglobin. Xu, Sheng,Hu, Bing,He, Ziyi,Ma, Fei,Feng, Jianfei,Shen, Wenbiao,Yang, Jie. 2011

[7]Genome-wide association study of seedling stage salinity tolerance in temperate japonica rice germplasm. Batayeva, Dariga,Dyuskalieva, Gulzhamal,Labaco, Benedick,Ye, Changrong,Vergara, Georgina,Reinke, Russell,Leung, Hei,Ye, Changrong,Li, Xiaolin,Usenbekov, Bakdaulet,Rysbekova, Aiman. 2018

[8]Applying a salinity response function and zoning saline land for three field crops: a case study in the Hetao Irrigation District, Inner Mongolia, China. Tong Wen-jie,Wen Xin-ya,Chen Fu,Zhang Hai-lin,Chu Qing-quan,Dikgwatlhe, Shadrack Batsile,Chen Xiao-li. 2015

[9]Larval development and salinity tolerance of Japanese flounder (Paralichthys olivaceus) from hatching to juvenile settlement. Wang, Youji,Guo, Qindan,Zhao, Hu,Lu, Weiqun,Liu, Haijin.

[10]Salinity tolerance as well as osmotic and ionic regulation in juvenile Chinese sturgeon (Acipenser sinensis Gray, 1835) exposed to different salinities. Zhao, F.,Qu, L.,Zhuang, P.,Zhang, L.,Liu, J.,Zhang, T.,Qu, L..

[11]Identification of salt-tolerant QTLs with strong genetic background effect using two sets of reciprocal introgression lines in rice. Cheng, Lirui,Wang, Yun,Meng, Lijun,Hu, Xia,Cui, Yanru,Sun, Yong,Zhu, Linghua,Xu, Jianlong,Li, Zhikang,Wang, Yun,Ali, Jauhar,Li, Zhikang.

[12]Co-expression of xerophyte Zygophyllum xanthoxylum ZxNHX and ZxVP1-1 confers enhanced salinity tolerance in chimeric sugar beet (Beta vulgaris L.). Wu, Guo-Qiang,Feng, Rui-Jun,Yuan, Hui-Jun,Wang, Suo-Min,Bao, Ai-Ke,Wei, Li,Wang, Chun-Mei. 2015

[13]Response of broomcorn millet (Panicum miliaceum L.) genotypes from semiarid regions of China to salt stress. Liu, Minxuan,Zhang, Shuang,Wang, Yinyue,Lu, Ping,Qiao, Zhijun,Wang, Yinyue. 2015

[14]Genetic diversity and association mapping for salinity tolerance in Bangladeshi rice landraces. Emon, Reza M.,Fan, Yeyang,Emon, Reza M.,Islam, Mirza M.,Halder, Jyotirmoy. 2015

[15]Growth response, carbohydrate and ion accumulation of diverse perennial ryegrass accessions to increasing salinity. Tang, Jinchi,Camberato, James J.,Yu, Xiaoqing,Jiang, Yiwei,Luo, Na,Bian, Shaomin. 2013

[16]Expressed sequence tag-simple sequence repeat-based molecular variance in two Salicornia (Amaranthaceae) populations. Xu, Z. L.,Ali, Z.,Yi, J. X.,He, X. L.,Zhang, D. Y.,Yu, G. H.,Ma, H. X.,Ali, Z.,Khan, A. A.,Ali, Z.,Khan, A. A.,Khan, I. A.. 2011

[17]HETEROLOGOUS EXPRESSION OF AN ALLIGATORWEED HIGH-AFFINITY POTASSIUM TRANSPORTER GENE ENHANCES SALINITY TOLERANCE IN ARABIDOPSIS THALIANA. Song, Zhizhong,Yang, Shunying,Jin, Man,Su, Yanhua,Song, Zhizhong,Yang, Shunying,Jin, Man,Zhu, Hong,Zhu, Hong. 2014

[18]Identification of salt tolerance-improving quantitative trait loci alleles from a salt-susceptible rice breeding line by introgression breeding. Qiu, Xianjin,Yuan, Zhihua,Liu, Huan,Yang, Longwei,He, Wenjing,Du, Bin,Xing, Danying,Xiang, Xiaojiao,Xu, Jianlong,Ye, Guoyou,Xu, Jianlong.

[19]Hydrogen-rich water alleviates salt stress in rice during seed germination. Xu, Sheng,Jiang, Yilong,Wang, Ning,Shen, Wenbiao,Xu, Sheng,Wang, Ren,Xu, Sheng,Wang, Ren,Zhu, Susong,Yang, Jie.

[20]Characterization of a wheat-Thinopyrum bessarabicum (T2JS-2BS center dot 2BL) translocation line. Qi, Zengjun,Du, Pei,Qian, Baoli,Zhuang, Lifang,Chen, Huafeng,Chen, Tingting,Shen, Jian,Guo, Jie,Feng, Yigao,Pei, Ziyou.

作者其他论文 更多>>

微信小程序