Precision SNP Genotyping for PVX Resistance Breeding in Potato: A Duplex Tetra-primer ARMS-PCR Approach Targeting the Rx1 and Rx2 Genes
文献类型： 外文期刊
第一作者： Islam, Saiful
作者： Islam, Saiful;Li, Jiana;Rahman, Mohammad Ataur;Xu, Binyan;Zheng, Jie;Zeng, Siyu;Song, Botao;Nie, Bihua;Song, Jiling
作者机构：
关键词： Tetraploid Potato; Potato Virus X; Resistance Gene; SNP Genotyping; Tetra-primer ARMS-PCR
期刊名称：AMERICAN JOURNAL OF POTATO RESEARCH （ 影响因子：1.8； 五年影响因子：2.3 ）
ISSN： 1099-209X
年卷期： 2025 年
页码：
收录情况： SCI
摘要： The Rx1 and Rx2 genes from wild potato species confer extreme resistance to potato virus X (PVX). Accurate genotyping is essential for their integration into breeding programs. We developed tetra-primer ARMS-PCR markers targeting SNPs (G2515C in Rx1 and C2445G in Rx2) in the LRR regions to distinguish resistance alleles. These markers effectively genotyped 50 tetraploid potato varieties, identifying 16 as Rx1 heterozygous, 5 as Rx2 heterozygous, and 29 as homozygous susceptible consistent with phenotypic data. The T-ARMS-PCR outperformed existing markers and enabled efficient duplex detection of both genes. Although no homozygous resistant varieties were found, artificial DNA mixtures validated marker reliability for homozygous and heterozygous resistance detection. To our knowledge, this is the first use of T-ARMS-PCR for Rx1/Rx2 genotyping, offering a high-throughput tool for marker-assisted selection in developing PVX-resistant cultivars.
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