High-Performance Fluorescent Microspheres Based on Fluorescence Resonance Energy Transfer Mode for Lateral Flow Immunoassays
文献类型: 外文期刊
第一作者: Wang, Yumeng
作者: Wang, Yumeng;Zhang, Gan;Xiao, Xiaoyue;Shu, Xinhui;Lai, Weihua;Fei, Dan;Guang, Yelan;Zhou, Yaomin
作者机构:
期刊名称:ANALYTICAL CHEMISTRY ( 影响因子:7.4; 五年影响因子:7.0 )
ISSN: 0003-2700
年卷期: 2023 年 95 卷 48 期
页码:
收录情况: SCI
摘要: The label with a large Stokes shift and strong fluorescence properties could improve the sensitivity of the lateral flow immunoassay (LFIA). Herein, two aggregation-induced emission (AIE) luminogens with spectral overlap were encapsulated in polymers by using the microemulsion method as a label, and the construction of a fluorescence resonance energy transfer mode was further verified via theoretical calculation and spectral analysis. Satisfactorily, the doped AIE polymer microspheres (DAIEPMs) exhibited a large Stokes shift of 285 nm and a 10.8-fold fluorescence enhancement compared to those of the AIEPMs loaded with acceptors. Benefiting from the excellent optical performance, DAIEPMs were applied to the LFIA for sensitive detection of chlorothalonil, which is an organochlorine pesticide. The limit of detection of the proposed DAIEPMs-LFIA was 1.2 pg/mL, which was 4.8-fold and 11.6-fold lower than those of quantum dot bead LFIA and gold nanoparticle LFIA, respectively. This work provides a new strategy to improve the optical properties of fluorescent materials and construct a sensitive and reliable detection platform.
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