A chlorogenic acid esterase from a metagenomic library with unique substrate specificity and its application in caffeic and ferulic acid production from agricultural byproducts

文献类型： 外文期刊

第一作者： Yao, Jian

作者： Yao, Jian;Gui, Lun;Long, Qizhang

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关键词： Metagenomic library; gene cloning; chlorogenic acid esterase; characterization

期刊名称：BIOCATALYSIS AND BIOTRANSFORMATION （ 影响因子：2.181； 五年影响因子：2.104 ）

ISSN： 1024-2422

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收录情况： SCI

摘要： Soil microbes are an abundant source of enzymes with unique properties that may be useful for industrial applications. As most wild-type strains show low chlorogenic acid esterase expression and activity, and most microbes cannot be cultured in the laboratory, a metagenomic approach provides methods of identifying new enzymes. In this study, a gene encoding a chlorogenic acid esterase, named Tan410, was isolated from a soil metagenomic library and overexpressed in Escherichia coli BL21 (DE3). The recombinant enzyme, with a predicted molecular weight of 54.88 kDa, was purified to homogeneity. The K-m and V-max values for Tan410 were 1.26 mM and 0.33 mM min(-1), respectively, with chlorogenic acid as the substrate. Its optimum temperature and pH for reaction were 30 degrees C and 7.5, respectively. The enzyme exhibited moderate thermostability and broad pH stability (3.0-10.0). Tan410 was also able to hydrolyse ethyl ferulate, methyl caffeate, propyl gallate, ethyl gallate, methyl vanillate, methyl benzoate, ethyl benzoate, methyl 2,5-dihydroxybenzoate, and methyl 3,5-dihydroxybenzoate, and it released caffeic and ferulic acids from agricultural byproducts (destarched wheat bran and coffee pulp). Tan140 has potential for industrial application in biomass valorization.

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