SALICYLIC ACID PRIMING PROMOTES SORGHUM GERMINATION UNDER DROUGHT STRESS: EVIDENCE FROM COMPARATIVE METABOLOMICS ANALYSIS

文献类型： 外文期刊

第一作者： Zhang, R. D.

作者： Zhang, R. D.;Chang, J. R.;Yue, Z. X.;Liang, X. H.;Cao, X.;Chang, J. R.;Yue, Z. X.;Zhou, Y. F.;Guo, W.

作者机构：

关键词： sorghum; salicylic acid; seed priming; drought stress; metabolomics

期刊名称：APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH （ 影响因子：0.7； 五年影响因子：0.9 ）

ISSN： 1589-1623

年卷期： 2023 年 21 卷 4 期

页码：

收录情况： SCI

摘要： Drought can significantly hinder seed germination and seedling emergence, even in drought tolerant crops like sorghum. Seed priming can improve seedling emergence under drought stress. Here, we sought to elucidate the mechanism by which seed priming with salicylic acid (SA) improves the drought tolerance of sorghum seedlings. We studied changes in morphology, antioxidant status, and primary metabolites in response to drought stress in both primed and unprimed seedlings. The experiment consisted of three treatment groups: normal germination (CK), unprimed seeds germinated under drought stress (D), and 0.1 mM SA-primed seeds germinated under drought stress (D-SA). The results showed that drought stress severely restrained sorghum seed germination, although SA mitigated the effects of drought stress. Compared to D, the shoot and root length increased 3.72-fold and 4.28-fold in D-SA, respectively. SA priming significantly increased the antioxidant enzyme activity and decreased the content of malondialdehyde (MDA) and H2O2 in sorghum seedlings under drought stress. A total of 518 metabolites were identified by ultra-high-performance liquid chromatography (UPLC) and tandem mass spectrometry (MS/MS), including 122 phenolic acids, 119 lipids, 89 amino acids and derivatives, 70 organic acids, 52 nucleotides and derivatives, and 66 others. Enrichment analysis showed that these metabolites were mainly involved in the stilbene, diarylheptane, and gingerol biosynthesis and flavonoid biosynthesis pathways. Among 68 core metabolites, phenolic acids and other substances within the phenylpropane biosynthesis pathway were enriched by seed priming with SA, including coniferin, chlorogenic acid, and sinapinaldehyde, among others. The phenolic acids may be the core metabolites influenced by exogenous SA, and may be at least partly responsible for the increased drought resistance exhibited by primed sorghum seedlings. The results of this study present an important step toward understanding the mechanism by which seed priming with SA increases the drought resistance of sorghum seedlings. It should be pointed out that the effect of SA priming on drought resistance of sorghum at other growth stages still needs further research.

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