Structure-function analysis of MhieJHAMT: a key enzyme in Monolepta hieroglyphica juvenile hormone biosynthesis

文献类型： 外文期刊

第一作者： Song, Xuan

作者： Song, Xuan;Zhang, Yong-jun;Song, Xuan;Yi, Chao-qun;Tang, Zi-yi;Liu, Wen-tao;Chen, Run;Guo, Wei-hao;Zhang, Tian-tao;Zhang, Yong-jun;Song, Xuan;Yang, Yong;Zhang, Da-wei;Zhang, Da-wei;Kou, Jun-feng;Liu, Chang;Tang, Zi-yi;Liu, Wen-tao

作者机构：

关键词： Monolepta hieroglyphica; Coleoptera pest; Juvenile hormone acid O-methyltransferase; Molecular dynamics; RNAi

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）

ISSN： 0141-8130

年卷期： 2025 年 318 卷

页码：

收录情况： SCI

摘要： Monolepta hieroglyphica, a major pest of Chinese crops, demands effective control. Targeting juvenile hormone acid O-methyltransferase, a key enzyme in JH production, presents a promising strategy for developing novel, specific insecticides. This study investigated the structure-function of M. hieroglyphica juvenile hormone acid Omethyltransferase (MhieJHAMT), a key enzyme in the JH pathway. Phylogenetic analysis showed MhieJHAMT's conservation within Coleoptera, suggesting a conserved catalytic mechanism. In vitro enzymatic activity assays confirmed MhieJHAMT's catalytic function. Bioinformatics (molecular dynamics and docking) revealed 19 amino acid residues forming the active site, binding S-adenosylmethionine (SAM) and farnesoic acid (FA). In vivo RNA interference (RNAi) coupled with ELISA demonstrated MhieJHAMT's crucial role in JH biosynthesis; its knockdown significantly reduced JH titers. Quantitative real-time PCR (RT-qPCR) revealed higher MhieJHAMT expression in female intestines and gonads, hinting at additional functions beyond JH biosynthesis. Interestingly, our findings reveal an intriguing correlation between host plant-mediated alterations in farnesyl pyrophosphate synthase 1 (MhieFPPS1) expression and that of MhieJHAMT, with MhieFPPS1 appearing to function upstream of MhieJHAMT. The observation suggests a potential positive feedback loop, hinting at a complex regulatory mechanism. This comprehensive study elucidates MhieJHAMT's function, its regulatory mechanisms, and its potential as a novel pest control target.

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