Cytochrome P450 CYP6AE70 Confers Resistance to Multiple Insecticides in a Lepidopteran Pest, Spodoptera exigua

文献类型： 外文期刊

第一作者： Hu, Bo

作者： Hu, Bo;Xing, Zhiping;Dong, Hui;Tan, Anjiang;Hu, Bo;Xing, Zhiping;Dong, Hui;Tan, Anjiang;Chen, Xiangzhu;Ren, Miaomiao;Liu, Kuitun;Rao, Cong;Su, Jianya

作者机构：

关键词： Spodoptera exigua; CYP450; insecticide resistance; resistance management; CYP6AE

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.2； 五年影响因子：6.4 ）

ISSN： 0021-8561

年卷期： 2024 年 72 卷 42 期

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收录情况： SCI

摘要： Cytochrome P450 monooxygenases are associated with the detoxification of xenobiotics, and overexpression of P450 genes has been proven to be associated with insecticide resistance in insect species. Our previous study has revealed that multiple CYP6AE genes were significantly overexpressed in a resistant strain of Spodoptera exigua, and among these genes, CYP6AE70 was particularly highly expressed. However, the functional roles of the CYP6AE genes in insecticide resistance remain unknown in this pest. Here, we investigate the relationship between the CYP6AE genes and insecticide resistance by focusing on CYP6AE70. The expression of CYP6AE70 was increased after exposure to chlorpyrifos, cypermethrin, and deltamethrin. Ectopic overexpression of P450 in transgenic flies by the GAL4/UAS system dramatically enhanced the tolerance to these three insecticides. Furthermore, the recombinant CYP6AE70 was functionally expressed in Sf9 cells, and metabolic assays revealed that the recombinant P450 protein could efficiently metabolize chlorpyrifos, cypermethrin, and deltamethrin. Finally, molecular modeling and docking also showed that this P450 protein were tightly bound to the three insecticides. These results determine that the upregulation of CYP6AE genes results in resistance to multiple insecticides in S. exigua. This study improves our understanding of P450-mediated insecticide resistance and will help us to design more effective resistance management for pest control.

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