Identification of a novel mutant allele, fzp-15, involved in panicle branch pattern of rice (Oryza sativa)

文献类型： 外文期刊

第一作者： Chen, Haiyuan

作者： Chen, Haiyuan;Zhang, Suobing;Tang, Weijie;Fang, Xianwen;Zhang, Yunhui;Cai, Yue;Zhang, Yunhui

作者机构：

关键词： advantageous alleles; CRISPR; FZP; panicle architecture; rice

期刊名称：PLANT BREEDING （ 影响因子：1.832； 五年影响因子：1.956 ）

ISSN： 0179-9541

年卷期： 2021 年 140 卷 4 期

页码：

收录情况： SCI

摘要： FRIZZY PANICLE (FZP) is involved in the development of panicle branching in rice. Herein, a novel mutant fzp-15, demonstrating the sequential rounds of branching and severe defects in SM (spikelet meristem) formation, was identified. Map-based cloning revealed that the abnormal panicle architecture of fzp-15 resulted from an amino acid substitution (Glu (E) to Ala (A)) in the N-terminal ERF domain of rice FZP protein. To generate new advantageous FZP alleles increasing grain numbers, CRISPR/Cas9 multiplex editing system was used to target the 5' untranslation region (UTR) of FZP in 'Nipponbare' (NIP). The 85-bp (-156 to -72 bp) or 149-bp (-157 to -9 bp) deletion in the upstream region of FZP can significantly increase grain numbers without a decrease in grain size, while the 38-bp deletion (-44 to -7 bp) in the upstream region of FZP has little effect in changing grain numbers. These results indicated that the 113-bp fragment (-157 to -45 bp) upstream of FZP is important to its function and deletion of this region can be used to increase grain numbers in rice.

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